eIF4EBP1 anticorps (truncated)

N° du produit ABIN2464007

L’anticorps Souris Monoclonal anti-eIF4EBP1 a été validé pour IHC. Il convient pour détecter eIF4EBP1 dans des échantillons de Humain.

Aperçu rapide pour eIF4EBP1 anticorps (truncated) (ABIN2464007)

Antigène: eIF4EBP1 (EIF4EBP1) (Eukaryotic Translation Initiation Factor 4E Binding Protein 1 (EIF4EBP1))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp eIF4EBP1 est non-conjugé

Application: Immunohistochemistry (IHC)

Clone: 11G12C11

Détail du produit anti-eIF4EBP1 anticorps

Épitope: truncated

Purification: Antibody is purified by protein A affinity chromatography.

Immunogène: Ni-NTA purified truncated recombinant 4EBP1 expressed in E. Coli strain BL21 (DE3).

Isotype: IgG1

Information d'application

Indications d'application: IHC:1:500 - 1:1,000
ELISA:Propose dilution 1:10,000.
Determining optimal working dilutions by titration test.

Restrictions: For Research Use only

Stockage

Buffer: Purified antibody in PBS containing 0.03 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Stock: -20 °C

Stockage commentaire: 4E-BP1 monoclonal antibody can be stored at -20 °C, stable for one year.

Détails sur eIF4EBP1

Antigène: eIF4EBP1 (EIF4EBP1) (Eukaryotic Translation Initiation Factor 4E Binding Protein 1 (EIF4EBP1))

Autre désignation: 4E-BP1

Sujet: 4E-BP1(eukaryotic translation Initiation Factor 4E Binding Protein 1),also called ELF4EBP1/BP-1/PHAS-I ,which is located on chromosome 8p12, with 118-amino acid protein (about 13 kDa). Binding of eIF4EBP1 to eIF4E is reversible and is dependent on the phosphorylation status of eIF4EBP1. Non phosphorylated eIF4EBP1 will bind strongly to eIF4E while(24 kDa), the phosphorylated form will not. Akt, TOR, MAP kinase, S6 kinase, and Cdc2 are known kinases capable of inactivating eIF4EBP1 binding to eIF4E by phosphorylating either threonines 35, 45, 69 or serine 64. Although, not all phosphorylation events equally block the eIF4EBP1-eIF4E interaction.

ID gène: 1978

UniProt: Q13541

Pathways: Signalisation MAPK, Signalisation PI3K-Akt, Signalisation RTK, AMPK Signaling, Regulation of Cell Size, BCR Signaling