CD74 anticorps (APC)

N° du produit ABIN2481707

L’anticorps Souris Monoclonal anti-CD74 a été validé pour FACS, WB, IF, IHC, IP et ICC. Il convient pour détecter CD74 dans des échantillons de Humain.

Aperçu rapide pour CD74 anticorps (APC) (ABIN2481707)

Antigène: CD74 (HLA-DR-gamma (CD74))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CD74 est conjugé à/à la APC

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Clone: PIN-1

Détail du produit anti-CD74 anticorps (APC)

Specificité: Detects ~33-35 kDa protein doublet corresponding to the molecular mass of the p33 and p35 forms of human CD74.

Réactivité croisée: Humain, Souris

Purification: Protein G Purified

Immunogène: Human CD74 invariant chain synthetic peptide

Isotype: IgG1

Information d'application

Indications d'application:

• WB (1:1000)
• IHC (1:100)
• ICC/IF (1:50)
• optimal dilutions for assays should be determined by the user.

Commentaires:

1 μg/ml of ABIN2481707 was sufficient for detection of CD74 in 20 μg of PALA cell lysates by colorimetric immunolot analysis using goat anti-mouse IgG: AP as the secondary antibody.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS pH 7.2, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Conjugated antibodies should be stored at 4°C

Détails sur CD74

Antigène: CD74 (HLA-DR-gamma (CD74))

Autre désignation: CD74

Sujet: CD74 is a non-polymorphic type II integral membrane protein. It has a short N-terminal cytoplasmic tail of 28 amino acids, followed by a single 24-aa transmembrane region and an approximately 150-aa lumenal domain (1). The CD74 chain is thought to function mainly as an MHC class II chaperone, which promotes ER exit of MHC class II molecules, directs them to endocytic compartments, prevents peptide binding in the ER, and contributes to peptide editing in the MHC class II compartment. Class II MHC and Ii expression was believed to be restricted to classical antigen-presenting cells (APC), however, during inflammation, other cell types, including mucosal epithelial cells, have also been reported to express class II MHC molecules (2). Experiments that investigate cell-surface CD74 are complicated by the fact that CD74 remains on the cell surface for a very short time. The surface half-life of CD74 was calculated to be fewer than 10 minutes (3). CD74 however has also recently been shown to have a role as an accessory-signaling molecule because of its high-affinity binding to the pro-inflammatory cytokine, macrophage migration-inhibitory factor (MIF) (3). The restricted expression of CD74 by normal tissues and its very rapid internalization make CD74 an attractive therapeutic target for both cancer and immunologic diseases (4).

ID gène: 972

NCBI Accession: NP_001020329

UniProt: P04233

Pathways: Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Negative Regulation of intrinsic apoptotic Signaling, Cancer Immune Checkpoints