LAMP1 anticorps (HRP)

N° du produit ABIN2484749

Cet anticorps anti-LAMP1 est un anticorps Souris Monoclonal détectant LAMP1 dans WB, IF, IP et ICC. Adapté pour Rat.

Aperçu rapide pour LAMP1 anticorps (HRP) (ABIN2484749)

Antigène: LAMP1 (Lysosomal-Associated Membrane Protein 1 (LAMP1))

Reactivité: Rat

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp LAMP1 est conjugé à/à la HRP

Application: Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Clone: Ly1C6

Détail du produit anti-LAMP1 anticorps (HRP)

Specificité: Detects ~120 kDa.

Réactivité croisée: Hamster, Humain, Souris, Rat

Purification: Protein G Purified

Immunogène: Rat liver lysosomal membrane preparations

Isotype: IgG1

Information d'application

Indications d'application:

• WB (1:1000)
• ICC/IF (1:1000)
• optimal dilutions for assays should be determined by the user.

Commentaires:

1 μg/ml was sufficient for detection of LAMP1 in rat liver miscrosome by ECL immunoblot analysis.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS pH 7.4, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Conjugated antibodies should be stored at 4°C

Détails sur LAMP1

Antigène: LAMP1 (Lysosomal-Associated Membrane Protein 1 (LAMP1))

Autre désignation: LAMP1

Sujet: Lysosme associated membrane proteins, or LAMP1 and LAMP2, are major constituents of the lysosomal membrane. The two have closely related structures, with 37 % sequence homology (2). They are both transmembrane glycoproteins that are localized primarily in lysosomes and late endosomes. Newly synthesized molecules are mostly transported from the trans-Golgi network directly to endosomes and then to lysosomes. A second pathway involves the lamps being delivered from the Golgi to the cell surface, and then along the endocytic pathway to the lysosomes. A minor pathway involves transport via the plasma membrane (3). Upon stimulation, a rapid translocation of intracellular LAMPs to the cell membrane is dependent on a carboxylterminal tyrosine ba based motif (YXXI) (1). If there is a disturbance in this spacing, lysosome localization of LAMP1 is abolished and the mutant protein then cycles between the membrane and the endosome (3). This stimulation has also been shown to have an associated release of histamine, leukotriene C (4) and prostaglandin D (2), which shows that LAMP-1 and LAMP-2 are activation markers for normal mast cells (1). They have also been linked to the inflammatory response in that they promote adhesion of human peripheral blood mononuclear cells (PBMC) to vascular endothelium, and therefore possibly the adhesion of PBMC to the site of inflammation (4).

ID gène: 25328

NCBI Accession: NP_036989

UniProt: P14562

Pathways: Autophagy