SOD2 anticorps (Biotin)

N° du produit ABIN2484764

Cet anticorps anti-SOD2 est un anticorps Lapin Polyclonal détectant SOD2 dans WB, IHC, ELISA, IP, IF et ICC. Adapté pour Rat.

Aperçu rapide pour SOD2 anticorps (Biotin) (ABIN2484764)

Antigène: SOD2 (Superoxide Dismutase 2, Mitochondrial (SOD2))

Reactivité: Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp SOD2 est conjugé à/à la Biotin

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunoprecipitation (IP), Immunofluorescence (IF), Immunocytochemistry (ICC)

Détail du produit anti-SOD2 anticorps (Biotin)

Specificité: Detects ~25 kDa.

Réactivité croisée: Poulet, Boeuf (Vache), Chien, Drosophila melanogaster, Cobaye, Hamster, Humain, Invertébré, Singe, Souris, Porc, Lapin, Rat, Sea Squirt, Mouton, Écureuil, Xenopus laevis

Purification: Protein A Purified

Immunogène: Recombinant Rat Mn SOD Protein

Information d'application

Indications d'application:

• WB (1:5000)
• IHC (1:100)
• ICC/IF (1:120)
• optimal dilutions for assays should be determined by the user.

Commentaires:

0.5 μg/ml of ABIN2484764 was sufficient for detection of Mn SOD in 20 μg of rat brain tissue extract by colorimetric immunoblot analysis using Goat anti-rabbit IgG:AP as the secondary antibody.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS pH 7.4, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Conjugated antibodies should be stored at 4°C

Détails sur SOD2

Antigène: SOD2 (Superoxide Dismutase 2, Mitochondrial (SOD2))

Autre désignation: SOD2

Sujet: Superoxide dismutase (SOD) is an endogenously produced intracellular enzyme present in almost every cell in the body (3). It works by catalyzing the dismutation of the superoxide radical O2ˉ to O2 and H2O2, which are then metabolized to H2O and O2 by catalase and glutathione peroxidase (2,5). In general, SODs play a major role in antioxidant defense mechanisms (4). There are two main types of SOD in mammalian cells. One form (SOD1) contains Cu and Zn ions as a homodimer and exists in the cytoplasm. The two subunits of 16 kDa each are linked by two cysteines forming an intra-subunit disulphide bridge (3). The second form (SOD2) is a manganese containing enzyme and resides in the mitochondrial matrix. It is a homotetramer of 80 kDa. The third form (SOD3 or EC-SOD) is like SOD1 in that it contains Cu and Zn ions, however it is distinct in that it is a homotetramer, with a mass of 30 kDA and it exists only in the extra-cellular space (7). SOD3 can also be distinguished by its heparin-binding capacity (1).

ID gène: 24787

NCBI Accession: NP_058747

UniProt: P07895

Pathways: Sensory Perception of Sound, Transition Metal Ion Homeostasis, Negative Regulation of intrinsic apoptotic Signaling