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DNMT1 anticorps (AA 637-650) (APC)

DNMT1 Reactivité: Humain WB, IHC, IP, ChIP Hôte: Souris Monoclonal 60B1220-1 APC
N° du produit ABIN2486004
  • Antigène Voir toutes DNMT1 Anticorps
    DNMT1 (DNA (Cytosine-5)-Methyltransferase 1 (DNMT1))
    Épitope
    • 26
    • 22
    • 16
    • 14
    • 12
    • 12
    • 10
    • 9
    • 8
    • 7
    • 7
    • 7
    • 6
    • 5
    • 4
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 637-650
    Reactivité
    • 203
    • 56
    • 46
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Humain
    Hôte
    • 156
    • 47
    • 1
    • 1
    Souris
    Clonalité
    • 144
    • 61
    Monoclonal
    Conjugué
    • 82
    • 17
    • 14
    • 12
    • 10
    • 9
    • 6
    • 6
    • 6
    • 6
    • 6
    • 6
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    Cet anticorp DNMT1 est conjugé à/à la APC
    Application
    • 133
    • 68
    • 45
    • 41
    • 33
    • 31
    • 28
    • 18
    • 17
    • 15
    • 14
    • 14
    • 5
    • 3
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Chromatin Immunoprecipitation (ChIP)
    Specificité
    Detects ~180 kDa. It will cross-react with mouse DNMT1.
     Réactivité croisée
    Poisson, Humain, Souris, Poisson zèbre (Danio rerio)
    Purification
    Protein G Purified
    Immunogène
    Raised against a synthetic peptide corresponding to amino acids 637-650 of human DNMT1
    Clone
    60B1220-1
    Isotype
    IgG1 kappa
    Top Product
    Discover our top product DNMT1 Anticorps primaire
  • Indications d'application
    • WB (1:1000)
    • IHC (1:1000)
    • optimal dilutions for assays should be determined by the user.
    Commentaires

    2 μg/ml of ABIN2486004 was sufficient for detection of Dnmt1 in 10 μg of mouse ES cell lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    1 mg/mL
    Buffer
    PBS, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated
    Agent conservateur
    Sodium azide
    Précaution d'utilisation
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Stock
    4 °C
    Stockage commentaire
    Conjugated antibodies should be stored at 4°C
  • Antigène
    DNMT1 (DNA (Cytosine-5)-Methyltransferase 1 (DNMT1))
    Autre désignation
    DNMT1 (DNMT1 Produits)
    Synonymes
    anticorps cb1075, anticorps cb983, anticorps dmnt1, anticorps fb05h01, anticorps mta, anticorps wu:fb05h01, anticorps ADCADN, anticorps AIM, anticorps CXXC9, anticorps DNMT, anticorps HSN1E, anticorps MCMT, anticorps DDM2, anticorps DECREASED DNA METHYLATION 2, anticorps DMT01, anticorps DMT1, anticorps DNA METHYLTRANSFERASE, anticorps DNA METHYLTRANSFERASE 01, anticorps DNA METHYLTRANSFERASE 1, anticorps MET2, anticorps METHYLTRANSFERASE 2, anticorps METHYLTRANSFERASE I, anticorps METI, anticorps methyltransferase 1, anticorps Cxxc9, anticorps Dnmt, anticorps Dnmt1o, anticorps MTase, anticorps Met-1, anticorps Met1, anticorps MommeD2, anticorps m.MmuI, anticorps DNA methyltransferase 1, anticorps DNA (cytosine-5-)-methyltransferase 1, anticorps methyltransferase 1, anticorps DNA methyltransferase (cytosine-5) 1, anticorps DNMT1, anticorps dnmt1, anticorps Dnmt1, anticorps MET1
    Sujet
    Methylation of DNA at cytosine residues plays an important role in regulation of gene expression, genomic imprinting and is essential for mammalian development. Hypermethylation of CpG islands in tumor suppressor genes or hypomethylation of bulk genomic DNA may be linked with development of cancer. To date, 3 families of mammalian DNA methyltransferase genes have been identified which include Dnmt1, Dnmt2 and Dnmt3. Dnmt1 is constitutively expressed in proliferating cells and inactivation of this gene causes global demethylation of genomic DNA and embryonic lethality. Dnmt2 is expressed at low levels in adult tissues and its inactivation does not affect DNA methylation or maintenance of methylation. The Dnmt3 family members, Dnmt3a and Dnmt3b, are strongly expressed in ES cells but their expression is down regulated in differentiating ES cells and is low in adult somatic tissue. Dnmt1 co-purifies with the retinoblastoma (Rb) tumour suppressor gene product, E2F1, and HDAC1. Dnmt1 also cooperates with Rb to repress transcription from promoters containing E2F-binding sites suggesting a link between DNA methylation, histone deacetylase and sequence-specific DNA binding activity, as well as a growth-regulatory pathway that is disrupted in nearly all cancer cells (1-6).
    ID gène
    1786
    NCBI Accession
    NP_001370
    UniProt
    P26358
    Pathways
    SARS-CoV-2 Protein Interactome, Phosphorylation & l'infection par le SRAS-CoV-2
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