CENPA anticorps (PE)

N° du produit ABIN2486500

L’anticorps Souris Monoclonal anti-CENPA a été validé pour WB, IF et ICC. Il convient pour détecter CENPA dans des échantillons de Humain.

Aperçu rapide pour CENPA anticorps (PE) (ABIN2486500)

Antigène: CENPA (Centromere Protein A (CENPA))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CENPA est conjugé à/à la PE

Application: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC)

Clone: 5A7-2E11

Détail du produit anti-CENPA anticorps (PE)

Specificité: Detects ~18 kDa. Recognizes Human CENP-A.

Réactivité croisée: Humain

Purification: Protein G Purified

Immunogène: Synthetic peptide corresponding to a portion of human CENP-A

Isotype: IgG1

Information d'application

Indications d'application:

• WB (1:1000)
• ICC/IF (1:100)
• optimal dilutions for assays should be determined by the user.

Commentaires:

1 μg/ml of ABIN2486500 was sufficient for detection of CENPA in 20 μg of U2OS cell lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Conjugated antibodies should be stored at 4°C

Détails sur CENPA

Antigène: CENPA (Centromere Protein A (CENPA))

Autre désignation: CENPA

Sujet: A replicated chromosome includes two kinetochores that control chromosome segregation during mitosis. The Centromere Protein-A, CENP-A, is a Histone H3-like protein that contains a C-terminal H3-like domain, which is required for centromere localization of CENP-A, and an antigenic N-terminal domain. CENP-A, originally isolated from HeLa cells, is essential for kinetochore targeting of CENP-C. In the presence of DNA CENP-A forms an octa-meric complex with histones H4, H2A, H2B. CENP-A specifically localizes to active centromeres and is a component of specialized centromeric nucleosomes, on which kinetochores are assembled. CENP-A is essential for nucleosomal packaging of centromeric DNA at interphase and functions as a centromere formation marker on the chromosome.

ID gène: 1058

NCBI Accession: NP_001035891

UniProt: P49450

Pathways: Chromatin Binding, Maintenance of Protein Location