Fibrinogen anticorps
Aperçu rapide pour Fibrinogen anticorps (ABIN2620902)
Antigène
Voir toutes Fibrinogen AnticorpsReactivité
Hôte
Clonalité
Conjugué
Application
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Specificité
- Mouse fibrinogen.
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Purification
- Purified IgG
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Immunogène
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Fibrinogen is isolated from fresh plasma after removing prothrombin. Freund's complete adjuvant is used in the first step of the immunization procedure.
Type of Immunogen: Purified protein
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Indications d'application
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Approved: DB, ELISA, IF
Usage: The reactivity of the antiserum is restricted to fibrinogen. In immunoelectrophoresis and radial immunodiffusion (Ouchterlony), using various antiserum concentrations against normal mouse plasma a single precipitin line is obtained which shows a reaction of identity with the precipitin line obtained with purified fibrinogen. No reaction is obtained with any other plasma protein component or serum. However, the antiserum may also react with fibrin monomers, circulating fibrinopeptides and fibrin degradation products. As unlabelled primary or secondary antibody reagent for the indirect detection of fibrinogen in mouse cells, tissues and body fluids in immunofluorescence and immunoenzyme methods. for the production of immunoconjugates with a selected marker. to prepare insoluble immunoaffinity adsorbents by coupling to an artificial carrier. as catching or detection reagent in non-isotopic methodology and solid phase immunochemistry (e. g. ELISA, Western blotting). When applied in any cytochemical or histochemical procedure or solids phase coupling technique, the optimum concentration of the IgG preparation should always be established by titration. Typical working dilutions in histochemistry are usually between 1:50 and 1:250. in ELISA and comparable non-precipitating antibody-binding assays between 1:500 and 1:5000. -
Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- Distilled water
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Concentration
- Lot specific
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Buffer
- Lyophilized from PBS, pH 7.2. No preservative added.
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Agent conservateur
- Without preservative
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Conseil sur la manipulation
- Avoid repeat freeze-thaw cycles.
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Stock
- 4 °C,-20 °C
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Stockage commentaire
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Long term: -20°C
Short term: +4°C. Avoid repeat freeze-thaw cycles.
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- Fibrinogen
Antigène
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