XBP1 anticorps (Internal Region)

N° du produit ABIN263217

Cet anticorps anti-XBP1 est un anticorps Chèvre Polyclonal détectant XBP1 dans WB, ELISA, IHC et IF. Adapté pour Humain.

Aperçu rapide pour XBP1 anticorps (Internal Region) (ABIN263217)

Antigène: XBP1 (X-Box Binding Protein 1 (XBP1))

Reactivité: Humain

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp XBP1 est non-conjugé

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Classe de qualité: Verified

Détail du produit anti-XBP1 anticorps

Épitope: Internal Region

Fonction: XBP1 / TREB5

Séquence: NHSWEDTFAN ELFPQ

Specificité: This antibody is expected to recognise isoform XBP1(S) (NP_001073007.1) only, The epitope for EB08557 is aa358-372.

Réactivité croisée: Chien, Humain, Souris, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogène: Peptide with sequence C-NHSWEDTFANELFPQ, from the internal region of the protein sequence according to NP_001073007.1.

Isotype: IgG

Information d'application

Indications d'application:

Immunohistochemistry: Paraffin embedded Human Breast. Recommended concentration: 5 μg/mL.

Western Blot: Approx 38 kDa band observed in Nuclear lysates of cell line HeLa (calculated MW of 40.1 kDa according to NP_001073007.1). Recommended concentration: 1-3 μg/mL. Primary incubation 1 hour.at room temperature.

Peptide ELISA: antibody detection limit dilution 1:4000.

Commentaires:

Immunofluorescence: Expression of the protein seen in the endoplasmic reticulum, cytoplasm and nucleus of HeLa and MCF7 cells. Recommended concentration: 10μg/ml.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Minimize freezing and thawing.

Stock: -20 °C

Stockage commentaire: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Détails sur XBP1

Antigène: XBP1 (X-Box Binding Protein 1 (XBP1))

Autre désignation: XBP1

Sujet: XBP1, TREB5, X-box binding protein 1 , XBP2, OTTHUMP00000028682, X-box-binding protein-1

ID gène: 7494, 22433

NCBI Accession: NP_001073007

Pathways: ER-Nucleus Signaling, Unfolded Protein Response