CD74 anticorps

N° du produit ABIN263954

L’anticorps anti-CD74 Monoclonal Souris est utilisé pour la détection de CD74 dans des échantillons de Humain. Il a été validé pour WB, IF, IP et IHC (fro).

Aperçu rapide pour CD74 anticorps (ABIN263954)

Antigène: CD74 (HLA-DR-gamma (CD74))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CD74 est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: PIN-1

Détail du produit anti-CD74 anticorps

Specificité: Detects ~33-35 kDa protein doublet, corresponding to the molecular mass of the p33 and p35 forms of human CD74 on SDS-PAGE immunoblots.

Attributs du produit: Synonyms: p33, DHLAG, HLA class II histocompatibility antigen gamma chain, HLA-DRantigens-associated invariant chain, Ia antigen-associated invariant chain

Purification: Purified

Immunogène: Human CD74 invariant chain synthetic peptide conjugated to KLH

Isotype: IgG1

Information d'application

Indications d'application: Western blot (1 μg/mL was sufficient for detection using colorimetric western blot analysis). Immunoprecipitation(5). Immunoflourescence(6). Flow cytometry. FACS (6).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: PBS, pH 7.2, 0.09 % azide and 50 % glycerol

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer. Avoidrepeated freezing and thawing.
Shelf life: one year from despatch.

Date de péremption: 12 months

Détails sur CD74

Antigène: CD74 (HLA-DR-gamma (CD74))

Autre désignation: CD74

Sujet: CD74 is a non-polymorphic type II integral membrane protein. It has a short N-terminal cytoplasmic tail of 28 amino acids, followed by a single 24-aa transmembrane region and an approximately 150-aa lumenal domain (1). The CD74 chain is thought to function mainly as an MHC class II chaperone, which promotes ER exit of MHC class II molecules, directs them to endocytic compartments, prevents peptide binding in the ER, and contributes to peptide editing in the MHC class II compartment. Class II MHC and Ii expression was believed to be restricted to classical antigen-presenting cells (APC), however, during inflammation, other cell types, including mucosal epithelial cells, have also been reported to express class II MHC molecules (2). Experiments that investigate cell-surface CD74 are complicated by the fact that CD74 remains on the cell surface for a very short time. The surface half-life of CD74 was calculated to be fewer than 10 minutes (3). CD74 however has also recently been shown to have a role as an accessory-signaling molecule because of its high-affinity binding to the pro-inflammatory cytokine, macrophage migration-inhibitory factor (MIF) (3). The restricted expression of CD74 by normal tissues and its very rapid internalization make CD74 an attractive therapeutic target for both cancer and immunologic diseases (4).Synonyms: DHLAG, HLA class II histocompatibility antigen gamma chain, HLA-DR antigens-associated invariant chain, Ia antigen-associated invariant chain, p33

ID gène: 972

UniProt: P04233

Pathways: Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Negative Regulation of intrinsic apoptotic Signaling, Cancer Immune Checkpoints