CD28 anticorps (APC)

N° du produit ABIN264248

Détail du produit anti-CD28 anticorps (APC)

Antigène: CD28

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CD28 est conjugé à/à la APC

Application: Flow Cytometry (FACS)

Specificité: This antibody recats with CD28 receptor.

Attributs du produit: Synonyms: TP44, T-cell-specific surface glycoprotein CD28

Clone: B-23

Isotype: IgG1

Information d'application

Indications d'application: Flow Cytometry. Monitoring of activated T cells in peripheral blood. Study of T lymphocyte cytokine function. Study of B cell activation. Study of Cell-adhesion molecules relating T and B lymphocytes.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Protocole: PBMC: Add 10 ul of MAB/10e6 PBMC in 100 ul PBS. Mix gently and incubate for 15 minutes at 2-8°C. Wash twice with PBS and analyze or fix with 0. 5% v/v of paraformaldehyde in PBS andanalyze.

Restrictions: For Research Use only

Stockage

Buffer: PBS containing 0.2 % protein carrier and 0.08 % Sodium Azide as preservative.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C. Do Not Freeze!
Shelf life: One year from despatch.

Date de péremption: 12 months

Détails sur CD28

Antigène: CD28

Autre désignation: CD28 (CD28 Produits)

Synonymes: anticorps Tp44, anticorps CD28RNA, anticorps cd28, anticorps CD28 molecule, anticorps CD28 antigen, anticorps Cd28 molecule, anticorps CD28 molecule L homeolog, anticorps CD28, anticorps Cd28, anticorps cd28.L

Sujet: Anti-human CD28 binds the 44 kDa MW cell surface protein on the surface of most T cells. CD28 acts as the ligand for the B7/BB-1 Molecule on the surface of activated B cells. B7/BB-1 costimulates T cells through CD28, along with CD2 and CD3.CD28 antigen is a disulfide-linked homodimeric glycoprotein. The CD28 antigen is present on approximately 60 % -80 % T lymphocytes (95 % of CD4 and 50 % of CD8 lymphocytes). CD28 regulates the expression of cytokines by T cells, not only IL-2, but also IL-1 alpha and CSF-1, usually synthesized by accessory cells. CD28 functions as a cell adhesion molecule (CAM) for certain T cell subsets.Synonyms: T-cell-specific surface glycoprotein CD28, TP44

ID gène: 940

UniProt: P10747

Pathways: TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response