KIT anticorps

N° du produit ABIN264306

Cet anticorps anti-KIT est un anticorps Monoclonal produit chez le Souris, détectant KIT dans FACS. Convient pour Humain.

Aperçu rapide pour KIT anticorps (ABIN264306)

Antigène: KIT (Mast/stem Cell Growth Factor Receptor (KIT))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp KIT est non-conjugé

Application: Flow Cytometry (FACS)

Clone: NU-c-kit

Détail du produit anti-KIT anticorps

Specificité: CD117 reacts against c-kit gene product (SCF receptor).

Attributs du produit: Synonyms: SCFR, KIT, Mast/stem cell growth factor receptor, Proto-Oncogene Tyrosine-Protein Kinase

Purification: Purified

Immunogène: Immunized with transfected cells with c-kit DNA in human leukemia cells (UT-7).

Isotype: IgG1

Information d'application

Indications d'application: Flow Cytometry. Monitoring of CD34-positive cells. Analysis of 8 % of myelomonocytes. Study of hematopoietic stem cell differentiation and proliferatiuon. Study of the c-kit gene product (SCF receptor). Study of a part of acute myeloblastic leukemia cells. Study of chronic myeloblastic leukemia cells.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Protocole: PBMC: Count the number of positive cells in peripheral blood. Add 10 µl of Mab to 50 µl suspended lymphocyte solution at 2 x 10e7. Mix gently and incubate for 15 minutes at 2-8°C.

Restrictions: For Research Use only

Stockage

Buffer: PBS containing 0.08 % Sodium Azide as preservative.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store the antibody (in aliquots) at -20 °C. Avoid repeated freezing and thawing.
Shelf life: One year from despatch.

Date de péremption: 12 months

Détails sur KIT

Antigène: KIT (Mast/stem Cell Growth Factor Receptor (KIT))

Autre désignation: CD117 / C-Kit

Sujet: CD117 reacts with 8 % of myelomonocytes and 75 % of CD34-positive cells. The hematopoietic stem cells are included in this fraction and therefore, this gene product is considered to have an important function for differentiation and proliferation of hematopoietic cells. CD117 also reacts with placenta, brain, a part of acute myeloblastic leukemia cells and chronic myeloblastic leukemia cells, but not with acute lymphoblastic leukemia cells. MW is 145 kDa.Synonyms: KIT, Mast/stem cell growth factor receptor, Proto-Oncogene Tyrosine-Protein Kinase, SCFR

ID gène: 3815

UniProt: P10721

Pathways: Signalisation RTK, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Sensory Perception of Sound, Stem Cell Maintenance, Production of Molecular Mediator of Immune Response, Regulation of long-term Neuronal Synaptic Plasticity