Cytokeratin 5 anticorps

N° du produit ABIN264415

Cet anticorps Souris Monoclonal détecte spécifiquement Cytokeratin 5 dans WB et IHC (fro). Il présente une réactivité avec des échantillons de Humain.

Aperçu rapide pour Cytokeratin 5 anticorps (ABIN264415)

Antigène: Cytokeratin 5 (KRT5) (Keratin 5 (KRT5))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Cytokeratin 5 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: 16-04-14

Détail du produit anti-Cytokeratin 5 anticorps

Réactivité croisée (Details): Also reacts to: KRT14

Attributs du produit: Synonyms: KRT5, Cytokeratin-5, Keratin-5, Keratin 5, CK5, K5, Keratin type II cytoskeletal 5, 58 kDaCytokeratin

Purification: Purified

Immunogène: Hybridoma produced by the fusion of splenocytes from mice immunized with cytokeratinenriched extract of Feline tongue epithelium and Mouse myeloma cells.

Isotype: IgG2a

Information d'application

Indications d'application: Western Blot: Detects a 58 kDa band, corresponding to Keratin 5, and a 50 kDa band,corresponding to Keratin 14. Immunohistochemistry on Frozen Sections: 1-5 μg/mL. Does not work on Paraffin embedded tissue sections.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 0.4 mg/mL

Buffer: PBS containing 0.08 % Sodium Azide as preservative.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store the antibody (in aliquots) at -20 °C. Can be shipped at 2-8 °C. Avoid repeated freezing and thawing.
Shelf life: One year from despatch.

Date de péremption: 12 months

Détails sur Cytokeratin 5

Antigène: Cytokeratin 5 (KRT5) (Keratin 5 (KRT5))

Autre désignation: Cytokeratin 5

Sujet: Cytokeratins (CK) are intermediate filaments of epithelial cells, both in keratinizing tissue (ie., skin) and non-keratinizing cells (ie., mesothelial cells). Although not a traditional marker for endothelial cells, cytokeratins have also been found in some microvascular endothelial cells. Atleast 20 different cytokeratins (CK) in the molecular range of 40-70 kDa and isoelectric points of 5-8.5 can be identified using two dimensional gel electrophoresis. Biochemically, most members of the CK family fall into one of two classes, type I (acidic polypeptides) and type II (basic polypeptides). At least one member of the acidic family and one member of the basic family is expressed in all epithelial cells. Monoclonal antibodies to cytokeratin proteins can be useful markers for tumor identification and classification. Belonging to the type A (acidic) subfamily of low molecular weight keratins and existing in combination with keratin 5, keratin 14 distinguishes stratified epithelial cells from simple epithelial cells and is useful in identification of squamous cell carcinomas. It is considered a prognostic marker in breast carcinomas.Synonyms: 58 kDa Cytokeratin, CK5, Cytokeratin-5, K5, KRT5, Keratin 5, Keratin type II cytoskeletal 5, Keratin-5

ID gène: 3852

UniProt: P13647