PLAT anticorps

N° du produit ABIN264767

L’anticorps anti-PLAT Polyclonal Lapin est utilisé pour la détection de PLAT dans des échantillons de Humain. Il a été validé pour WB, IHC (fro) et EIA.

Aperçu rapide pour PLAT anticorps (ABIN264767)

Antigène: PLAT (Plasminogen Activator, Tissue (PLAT))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp PLAT est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA)

Détail du produit anti-PLAT anticorps

Specificité: This antibody recognizes tPA.

Purification: Protein A Chromatography

Immunogène: Recombinant human tPA

Information d'application

Indications d'application: ELISA: 1: 10,000 - 1: 100,000. Western blot: 1: 1,000 - 1: 10,000. Immunohistochemistry: 1: 100 - 1: 1,000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 4.5 mg/mL (OD280nm, E0.1% = 1.36)

Buffer: 0.05 M Sodium Phosphate, pH 6.6 containing 0.5 M Sodium Chloride and 1 mM EDTA

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: -80 °C

Détails sur PLAT

Antigène: PLAT (Plasminogen Activator, Tissue (PLAT))

Autre désignation: T-PA / PLAT

Sujet: TPA converts the abundant, but inactive, zymogen plasminogen to plasmin by hydrolyzing a single Arg-Val bond. By controlling plasmin-mediated proteolysis, it plays an important role in tissue remodeling and degradation, cell migration and many other physiopathological events. TPA binds to fibrin with high affinity, this leads to an increase in the catalytic efficiency of the enzyme of 100- to 1000-fold, due to an increase in affinity for plasminogen. Similarly, binding to heparin increases the activation of plasminogen. Binding to laminin and fibronectin has also been demonstrated. TPA also binds to mannose receptor and the low density lipoprotein receptor related protein (LRP1). These proteins are involved in TPA clearance. TPA binds to annexin II and to cytokeratin 8. As yet unidentified interactions on endothelial cells and vascular smooth muscle cells (VSMC) lead to a 100-fold stimulation of plasminogen activation. Binding to VSMC reduces TPA inhibition by PAI-1 by 30-fold.Synonyms: Alteplase, Reteplase, Tissue-type plasminogen activator, tPA

ID gène: 5327

NCBI Accession: NP_000921

UniProt: P00750

Pathways: Autophagy, Smooth Muscle Cell Migration, Platelet-derived growth Factor Receptor Signaling, SARS-CoV-2 Protein Interactome