SIAH1 anticorps

N° du produit ABIN265176

Cet anticorps anti-SIAH1 Polyclonal Lapin (ABIN265176) détecte spécifiquement SIAH1 dans WB. L’anticorps est réactif avec des échantillons de Humain.

Aperçu rapide pour SIAH1 anticorps (ABIN265176)

Antigène: SIAH1 (E3 Ubiquitin-Protein Ligase SIAH1 (SIAH1))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp SIAH1 est non-conjugé

Application: Western Blotting (WB)

Détail du produit anti-SIAH1 anticorps

Specificité: This antibody detects SIAH1.

Purification: Affinity chromatography

Immunogène: Synthetic peptide derived from the human SIAH-1 protein

Isotype: IgG

Information d'application

Indications d'application: Western blot: 1-5 μg/mL). Positive control: human brain lysate.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Buffer: Phosphate buffered saline with 0.08 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: -20 °C

Stockage commentaire: Ship on dry ice. Upon arrival, aliquot and freeze at -20 °C to -70 °C.

Détails sur SIAH1

Antigène: SIAH1 (E3 Ubiquitin-Protein Ligase SIAH1 (SIAH1))

Autre désignation: SIAH1

Sujet: Seven in absentia homolog 1 (SIAH-1) is a member of the RING-finger-containing E3 ubiquitin ligases. Alpha-synuclein and synphilin-1 are substrates of SIAH-1. Both proteins are involved in the development of Parkinson's disease (PD). Mutations in Parkin, another E3 ubiquitin ligase which ubiquinates synphilin-1 and glycosylated alpha-synuclein, have been defined as a major cause of autosomal recessive PD. The role of SIAH-1 in PD is highlighted by the fact that SIAH-1 is a component of the Lewy bodies and plays a role in apoptosis caused by nitric oxide (NO) induced oxidative stress. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) a classic glycolytic enzyme, and multi-functional protein. GAPDH plays role as a mediator for cell death. GAPDH translocates to the nucleus under a variety of stressors/conditions, most of which are associated with oxidative stress. Sequential steps lead to nuclear translocation of GAPDH during cell death, 1] a catalytic cysteine in GAPDH (C150 in rat GAPDH) is S-nitrosylated by nitric oxide (NO) which is generated from inducible nitric oxide synthase (iNOS) and/or neuronal NOS (nNOS), 2] the modified GAPDH becomes capable of binding with Siah1, an E3 ubiquitin ligase, and stabilizes it,3] the GAPDH-Siah protein complex translocates to the nucleus, dependent on Siah1's nuclear localization signal, and degrades Siah1's substrates in the nucleus, which results in cytotoxicity.Synonyms: E3 ubiquitin-protein ligase SIAH1, HUMSIAH, Seven in absentia homolog 1, Siah-1, Siah-1a

ID gène: 6477

NCBI Accession: NP_001006611

UniProt: Q8IUQ4