Cullin 1 anticorps

N° du produit ABIN265375

Détail du produit anti-Cullin 1 anticorps

Antigène: Cullin 1 (CUL1)

Reactivité: Humain, Souris

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Cullin 1 est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Specificité: This antibody detects endogenous levels of Cullin-1 protein (region surrounding Tyr761).

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Pureté: > 95 % pure by SDS-PAGE

Information d'application

Indications d'application: ELISA: 1: 20000approx. 1: 40000. WB: 1: 500approx. 1: 1000. IHC: 1: 50approx. 1: 200. IF: 1: 50approx. 1: 200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.2., 0.05 % Sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Détails sur Cullin 1

Antigène: Cullin 1 (CUL1)

Autre désignation: Cullin 1 (CUL1 Produits)

Synonymes: anticorps LOC100282292, anticorps Cullin-1, anticorps Cul1, anticorps zgc:55747, anticorps zgc:77500, anticorps wu:fc13a10, anticorps cullin-1, anticorps ATCUL1, anticorps AUXIN RESISTANT 6, anticorps AXR6, anticorps T10P11.26, anticorps T10P11_26, anticorps cullin 1, anticorps cul1, anticorps wu:fi43h07, anticorps wu:fj43a08, anticorps zgc:111899, anticorps zgc:55483, anticorps Cul-1, anticorps cullin 1, anticorps cullin-1, anticorps Cullin 1, anticorps Cullin-1, anticorps cullin 1a, anticorps cullin 1 S homeolog, anticorps cullin 1b, anticorps CUL1, anticorps LOC100282292, anticorps cul1, anticorps ATEG_00491, anticorps EDI_072700, anticorps EDI_264400, anticorps SJAG_02591, anticorps BDBG_03785, anticorps Cul1, anticorps cul1a, anticorps cul1.S, anticorps cul-1, anticorps cul1b

Sujet: CUL-1, which is the mammalian homolog of yeast Cdc53, is an integral component of the E3 ubiquitin ligase complex designated SCF. The SCF (Skp1/CUL-1/F-box protein complex) consists of Skp1 associating with both CUL-1 and an F-box protein, such as Skp2, which determines the substrate specificity of the complex. CUL-1-mediated ubiquitination results in the degradation of cell cycle proteins cyclin D, p21 and cyclin E. Another cullin, CUL-3, facilitates the degradation of cyclin E independent of SCF activity, while CUL-2 associates with the tumor suppressing protein VHL and elongin B to form VBC complexes, which structurally resemble the SCF ligase.Synonyms: CUL-1, CUL1, Cullin-1

Poids moléculaire: approx. 90 kDa

ID gène: 8454

NCBI Accession: NP_003583

UniProt: Q13616

Pathways: Cycle Cellulaire, Signalisation Hedgehog, Mitotic G1-G1/S Phases, Regulation of Cell Size