GRP94 anticorps

N° du produit ABIN265448

Détail du produit anti-GRP94 anticorps

Antigène: GRP94 (HSP90B1) (Heat Shock Protein 90kDa beta (Grp94), Member 1 (HSP90B1))

Reactivité: Humain, Souris

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp GRP94 est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Specificité: This antibody detects endogenous levels of GRP94/HSP90B1 protein. (region surrounding Glu790)

Réactivité croisée (Details): Species reactivity (tested):Human, Mouse.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Pureté: > 95 % pure by SDS-PAGE

Information d'application

Indications d'application: ELISA: 1: 5000approx. 1: 20000. WB: 1: 500approx. 1: 1000. IHC: 1: 50approx. 1: 200. IF: 1: 50approx. 1: 200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.2., 15 mM sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: DO NOT FREEZE!

Stock: 4 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C.

Détails sur GRP94

Antigène: GRP94 (HSP90B1) (Heat Shock Protein 90kDa beta (Grp94), Member 1 (HSP90B1))

Autre désignation: Endoplasmin / HSP90B1 / TRA1 (HSP90B1 Produits)

Synonymes: anticorps ecgp, anticorps gp96, anticorps tra1, anticorps grp94, anticorps DDBDRAFT_0206450, anticorps DDBDRAFT_0215015, anticorps DDB_0206450, anticorps DDB_0215015, anticorps TRA1, anticorps HSP90B1, anticorps ERp99, anticorps GRP94, anticorps TA-3, anticorps Targ2, anticorps Tra-1, anticorps Tra1, anticorps endoplasmin, anticorps GP96, anticorps fb61d09, anticorps tra-1, anticorps wu:fb61d09, anticorps wu:fq25g01, anticorps Grp94, anticorps ECGP, anticorps Gp96, anticorps ppk98, anticorps hsp108, anticorps heat shock protein 90kDa beta (Grp94), member 1, anticorps heat shock protein Hsp90 family protein, anticorps 94 kD glucose-regulated protein, anticorps heat shock protein 90 beta family member 1, anticorps heat shock protein 90, beta (Grp94), member 1, anticorps heat shock protein 90, beta (grp94), member 1, anticorps heat shock protein 90kDa beta family member 1 L homeolog, anticorps hsp90b1, anticorps grp94, anticorps HSP90B1, anticorps Hsp90b1, anticorps hsp90b1.L

Sujet: Glucose regulated protein 94 (GRP 94) is a resident protein of the endoplasmic reticulum (ER) and is induced by the accumulation of unfolded proteins suggesting that it might associate transiently with a variety of newly synthesized secretory and membrane proteins or permanently with mutant or defective proteins. The highly conserved sequence Lys-Asp-Glu-Leu (KDEL) is present at the C terminus of GRP 94 and other resident ER proteins including GRP 78 and protein disulfide isomerase (PDI). The presence of carboxy terminal KDEL appears to be necessary for retention and appears to be sufficient to reduce the secretion of proteins from the ER. This retention is reported to be mediated by a KDEL receptor. GRP 94 is also a low affinity, high capacity calcium binding protein, though it's role, if any, in calcium regulation is not understood.Synonyms: 94 kDa glucose-regulated protein, GRP94, Heat shock protein 90 kDa beta member 1, Tumor rejection antigen 1

Poids moléculaire: approx. 94 kDa

ID gène: 7184

NCBI Accession: NP_003290

UniProt: P14625

Pathways: Thyroid Hormone Synthesis, Activation of Innate immune Response, ER-Nucleus Signaling, Toll-Like Receptors Cascades