AGO1 anticorps (N-Term)

N° du produit ABIN2668226

Cité dans 2 publications. Cet anticorps anti-AGO1 Monoclonal Rat (Clone 4B8) (ABIN2668226) détecte spécifiquement AGO1 dans WB et IP. L’anticorps est réactif avec des échantillons de Humain.

Aperçu rapide pour AGO1 anticorps (N-Term) (ABIN2668226)

Antigène: AGO1 (EIF2C1) (Eukaryotic Translation Initiation Factor 2C, 1 (EIF2C1))

Reactivité: Humain

Hôte: Rat

Clonalité: Monoclonal

Conjugué: Cet anticorp AGO1 est non-conjugé

Application: Western Blotting (WB), Immunoprecipitation (IP)

Clone: 4B8

Détail du produit anti-AGO1 anticorps

Épitope: N-Term

Fonction: Ago1 antibody (mAb) (Clone 4B8)

Purification: Protein G Chromatography

Immunogène: This Ago1 antibody was raised against a peptide from the N-terminus of human Argonaute 1 (Ago1) protein.

Isotype: IgG2a

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Stockage

Concentration: 1 μg/μL

Buffer: Purified IgG in 70 mM Tris ( pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30 % glycerol and 0.035 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

Stock: -20 °C

Stockage commentaire: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Date de péremption: 24 months

Références pour anticorps anti-AGO1 (ABIN2668226)

Beitzinger, Peters, Zhu, Kremmer, Meister: "Identification of human microRNA targets from isolated argonaute protein complexes." dans: RNA biology, Vol. 4, Issue 2, pp. 76-84, (2007) (PubMed).

Höck, Weinmann, Ender, Rüdel, Kremmer, Raabe, Urlaub, Meister: "Proteomic and functional analysis of Argonaute-containing mRNA-protein complexes in human cells." dans: EMBO reports, Vol. 8, Issue 11, pp. 1052-60, (2007) (PubMed).

Détails sur AGO1

Antigène: AGO1 (EIF2C1) (Eukaryotic Translation Initiation Factor 2C, 1 (EIF2C1))

Autre désignation: Ago1

Sujet: The Argonaute family proteins (Ago1, Ago2, Ago3 and Ago4) are involved in RNAi mediated gene silencing through siRNA and miRNA effectors. The Argonaute proteins are part of RISC, the RNAi Induced Silencing Complex. Binding of RISC can inhibit mRNA translation and results in the degradation of the mRNA through the action of Ago2-mediated target cleavage. Ago1 is non-essential for siRNA-directed target RNA cleavage but is needed for the production of mature miRNA that affects miRNA-directed RNA cleavage. Argonaute family proteins contain a PAZ and a PIWI domain.

Poids moléculaire: 100 kDa

ID gène: 26523

NCBI Accession: NP_036331

Pathways: Fc-epsilon Receptor Signaling Pathway, Regulatory RNA Pathways, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Hormone Transport, Regulation of Actin Filament Polymerization, Stem Cell Maintenance, Ribonucleoprotein Complex Subunit Organization