DNMT3B anticorps (AA 1-218)

N° du produit ABIN2668253

Cité dans 1 publication. Cet anticorps anti-DNMT3B Polyclonal Lapin (ABIN2668253) détecte spécifiquement DNMT3B dans WB. L’anticorps est réactif avec des échantillons de Souris.

Aperçu rapide pour DNMT3B anticorps (AA 1-218) (ABIN2668253)

Antigène: DNMT3B (DNA (Cytosine-5-)-Methyltransferase 3 beta (DNMT3B))

Reactivité: Souris

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp DNMT3B est non-conjugé

Application: Western Blotting (WB)

Détail du produit anti-DNMT3B anticorps

Épitope: AA 1-218

Fonction: DNMT3B antibody (pAb)

Purification: Affinity Purified

Immunogène: This DNMT3B antibody was raised against a recombinant protein corresponding to amino acids 1-218 of mouse DNMT3B.

Isotype: IgG

Information d'application

Indications d'application: WB*: 1:500 - 1:1,000 dilution *Note: many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt / Sonication Protocol when preparing nuclear extracts for Western blot.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: Purified IgG in 70 mM Tris ( pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30 % glycerol and 0.035 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

Stock: -20 °C

Stockage commentaire: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Date de péremption: 24 months

Références pour anticorps anti-DNMT3B (ABIN2668253)

Ajj, Chesnel, Pinel, Plenat, Flament, Dumond: "An alkylphenol mix promotes seminoma derived cell proliferation through an ERalpha36-mediated mechanism." dans: PLoS ONE, Vol. 8, Issue 4, pp. e61758, (2013) (PubMed).

Détails sur DNMT3B

Antigène: DNMT3B (DNA (Cytosine-5-)-Methyltransferase 3 beta (DNMT3B))

Autre désignation: DNMT3B

Sujet: DNMT3B (DNA (Cytosine-5-)-Methyltransferase 3 Beta) proteins are involved in DNA methylation in which a methyl group is added to a cytosine residue on DNA, commonly at the C5 position of a CpG dinucleotide. Three families of DNMTs have been identified: DNMT1, DNMT2 and DNMT3. The DNMT3 family contains two active methyltransferases, DNMT3A & DNMT3B, and one DNMT3-Like protein (DNMT3L). DNMT3B has been shown to be important in the regulation of specific patterns of DNA methylation, specifically de novo methylation. Methylation of mammalian DNA has long been recognized to play a major role in a number of cellular functions such as embryonic development, genetic imprinting, X chromosome inactivation and the control of gene expression. DNA methylation is generally associated with transcriptional repression. Mutations in the DNMT3B gene cause the immunodeficiency-centromeric instability-facial anomalies (ICF) syndrome.

Poids moléculaire: 110 kDa

ID gène: 13436

NCBI Accession: NP_001003960