SIRT1 anticorps (AA 581-630)

N° du produit ABIN2668278

Cet anticorps Souris Monoclonal détecte spécifiquement SIRT1 dans WB, IP, ICC et IF. Il présente une réactivité avec des échantillons de Humain. Il a été cité dans 2+ publications.

Aperçu rapide pour SIRT1 anticorps (AA 581-630) (ABIN2668278)

Antigène: SIRT1 (Sirtuin 1 (SIRT1))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp SIRT1 est non-conjugé

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunocytochemistry (ICC), Immunofluorescence (IF)

Clone: 2G1-F7

Détail du produit anti-SIRT1 anticorps

Épitope: AA 581-630

Fonction: SIRT1 antibody (mAb) (Clone 2G1/F7)

Purification: Protein G Chromatography

Immunogène: This SIRT1 antibody was raised against a synthetic peptide corresponding to amino acids 581-630 of human SIRT1.

Isotype: IgG1

Information d'application

Indications d'application: WB: 0.5 - 2.0 μg/mL dilution The addition of 0.05 % Tween 20 in the blocking buffer and primary antibody incubation buffer is recommended to aid in detection by Western blot. Individual optimization may be required.

Restrictions: For Research Use only

Stockage

Concentration: 1 μg/μL

Buffer: 10 mM sodium phosphate pH 7.5, 150 mM NaCl, 30 % glycerol, 0.035 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

Stock: -20 °C

Stockage commentaire: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Date de péremption: 24 months

Références pour anticorps anti-SIRT1 (ABIN2668278)

Rajendran, Kidane, Yu, Dashwood, Bisson, Löhr, Ho, Williams, Dashwood: "HDAC turnover, CtIP acetylation and dysregulated DNA damage signaling in colon cancer cells treated with sulforaphane and related dietary isothiocyanates." dans: Epigenetics, Vol. 8, Issue 6, pp. 612-23, (2013) (PubMed).

Vaquero, Scher, Lee, Erdjument-Bromage, Tempst, Reinberg: "Human SirT1 interacts with histone H1 and promotes formation of facultative heterochromatin." dans: Molecular cell, Vol. 16, Issue 1, pp. 93-105, (2004) (PubMed).

Détails sur SIRT1

Antigène: SIRT1 (Sirtuin 1 (SIRT1))

Autre désignation: SIRT1

Sujet: SIRT1 (Sirtuin 1, SIR2L1) is a member of the Sirtuin family of proteins related to the yeast Sir2 protein. Like Sir2 and other Sirtuins, SIRT1 is an NAD-dependent histone and protein deacetylase. SIRT1 catalyzes the removal of acetyl groups from a variety of protein substrates (NBS1, p53, Ku70, etc.) as well as from Histone H4 Lys16. SIRT1 is involved in the regulation of a number of cellular processes, including transcription, metabolism, DNA repair and aging, and increased SIRT1 is associated with extended lifespan of C. elegans.

Poids moléculaire: 120 kDa

ID gène: 23411

NCBI Accession: NP_036370

Pathways: Signalisation MAPK, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Carbohydrate Homeostasis, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process, Positive Regulation of Response to DNA Damage Stimulus, Negative Regulation of intrinsic apoptotic Signaling