KDM4A anticorps (AA 586-674)

N° du produit ABIN2668289

Cet anticorps anti-KDM4A Monoclonal Souris (Clone N154-32) (ABIN2668289) détecte spécifiquement KDM4A dans WB. L’anticorps est réactif avec des échantillons de Humain et Souris.

Aperçu rapide pour KDM4A anticorps (AA 586-674) (ABIN2668289)

Antigène: KDM4A (Lysine (K)-Specific Demethylase 4A (KDM4A))

Reactivité: Humain, Souris

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp KDM4A est non-conjugé

Application: Western Blotting (WB)

Clone: N154-32

Détail du produit anti-KDM4A anticorps

Épitope: AA 586-674

Fonction: JMJD2A antibody (mAb) (Clone N154/32)

Purification: Protein A Chromatography

Immunogène: This JMJD2A antibody was raised against a recombinant protein corresponding to amino acids 586-674 of human JMJD2A.

Isotype: IgG1

Information d'application

Indications d'application: WB*: 1 - 2 μg/mL dilution *Note: many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt / Sonication Protocol when preparing nuclear extracts for Western blot. The addition of 0.05 % Tween 20 in the blocking buffer and primary antibody incubation buffer is also recommended to aid in detection by Western blot. This antibody is also available as an AbFlex engineered recombinant antibody. For details on the corresponding AbFlex Recombinant Antibody, see Catalog No. 91143.

Restrictions: For Research Use only

Stockage

Concentration: 1 μg/μL

Buffer: Purified IgG in PBS with 30 % glycerol and 0.035 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

Stock: -20 °C

Stockage commentaire: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Date de péremption: 24 months

Détails sur KDM4A

Antigène: KDM4A (Lysine (K)-Specific Demethylase 4A (KDM4A))

Autre désignation: JMJD2A

Sujet: JMJD2A (KDM4A, JHDM3A) is a member of the Jmj-containing (Jumonji) class of histone demethylase proteins that are involved in the regulation of genome function through the removal of methyl groups from histones. JMJD2A specifically demethylates histone H3 dimethyl lysine 9 (H3K9me2), trimethyl lysine 9, (H3K9me3), dimethyl lysine 36 (H3K36me2) and trimethyl lysine 36 (H3K36me3). It also participates in transcriptional repression of ASCL2 and E2F-responsive promoters via the recruitment of histone deacetylases and NCOR1. JMJD2A contains JmjC and JmjN domains, two PHD fingers and two tudor domains.

Poids moléculaire: 130 kDa

ID gène: 9682

NCBI Accession: NP_055478

Pathways: L'effet Warburg