DNMT1 anticorps (AA 1-110)

N° du produit ABIN2668501

Il existe 1 publication pour ce produit. L’anticorps anti-DNMT1 Polyclonal Lapin est utilisé pour la détection de DNMT1 dans des échantillons de Humain. Il a été validé pour WB, IP et IF.

Aperçu rapide pour DNMT1 anticorps (AA 1-110) (ABIN2668501)

Antigène: DNMT1 (DNA (Cytosine-5)-Methyltransferase 1 (DNMT1))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp DNMT1 est non-conjugé

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunofluorescence (IF)

Détail du produit anti-DNMT1 anticorps

Épitope: AA 1-110

Fonction: DNMT1 antibody (pAb)

Purification: Protein A Chromatography

Immunogène: This DNMT1 antibody was raised against a recombinant protein corresponding to amino acids 1-110 of human DNMT1.

Isotype: IgG

Information d'application

Indications d'application: IP: 10 μg per IP WB*: 0.5 - 2 μg/mL dilution IF: 1:500 dilution *Note: many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt / Sonication Protocol when preparing nuclear extracts for Western Blot. For DNMT1, we also offer AbFlex DNMT1 Recombinant Antibody (rAb). For details, see Catalog No. 91175.

Restrictions: For Research Use only

Stockage

Concentration: 1 μg/μL

Buffer: Purified IgG in PBS with 30 % glycerol and 0.035 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

Stock: -20 °C

Stockage commentaire: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Date de péremption: 24 months

Références pour anticorps anti-DNMT1 (ABIN2668501)

Londoño Gentile, Lu, Lodato, Tse, Olejniczak, Witze, Thompson, Wellen: "DNMT1 is regulated by ATP-citrate lyase and maintains methylation patterns during adipocyte differentiation." dans: Molecular and cellular biology, Vol. 33, Issue 19, pp. 3864-78, (2013) (PubMed).

Détails sur DNMT1

Antigène: DNMT1 (DNA (Cytosine-5)-Methyltransferase 1 (DNMT1))

Autre désignation: DNMT1

Sujet: DNMT1 (DNA (cytosine-5)-methyltransferase) proteins are involved in DNA methylation in which a methyl group is added to a cytosine residue on DNA, commonly at the C5 position of a CpG dinucleotide. Three families of DNMTs have been identified: DNMT1, DNMT2, and DNMT3 (comprised of DNMT3A and DNMT3B). DNMT1 is the most abundant DNMT in somatic cells and shows a preference for methylating hemi-methylated DNA. It is considered to be a maintenance DNA methyltransferase that is important in the maintenance of specific patterns of methylation throughout cellular divisions. Methylation of mammalian DNA has long been recognized to play a major role in a number of cellular functions such as embryonic development, genetic imprinting, X chromosome inactivation and the control of gene expression. DNA methylation is generally associated with transcriptional repression.

Poids moléculaire: 185 kDa

ID gène: 1786

NCBI Accession: NP_001370

Pathways: SARS-CoV-2 Protein Interactome, Phosphorylation & l'infection par le SRAS-CoV-2