Rpb1 CTD anticorps (pSer7)

N° du produit ABIN2668542

Cet anticorps Rat Monoclonal détecte spécifiquement Rpb1 CTD dans WB, IF, ICC, ChIP, ChIP-seq et IP. Il présente une réactivité avec des échantillons de Humain. Il a été cité dans 6+ publications.

Aperçu rapide pour Rpb1 CTD anticorps (pSer7) (ABIN2668542)

Antigène: Rpb1 CTD (RNA Polymerase II CTD)

Reactivité: Humain

Hôte: Rat

Clonalité: Monoclonal

Conjugué: Cet anticorp Rpb1 CTD est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), Chromatin Immunoprecipitation (ChIP), ChIP DNA-Sequencing (ChIP-seq), Immunoprecipitation (IP)

Clone: 4E12

Détail du produit anti-Rpb1 CTD anticorps

Épitope: pSer7

Fonction: RNA pol II CTD phospho Ser7 antibody (mAb) (Clone 4E12)

Purification: Protein G Chromatography

Immunogène: This RNA pol II CTD phospho Ser7 antibody was raised against a peptide containing the RNA pol II CTD sequence phosphorylated at serine 7.

Isotype: IgG1

Information d'application

Indications d'application: ChIP: 5-10 μg per ChIP ChIP-Seq: 5-10 μg each

Restrictions: For Research Use only

Stockage

Concentration: 1 μg/μL

Buffer: Purified IgG in PBS, 30 % glycerol and 0.035 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

Stock: -20 °C

Stockage commentaire: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Date de péremption: 24 months

Références pour anticorps anti-Rpb1 CTD (ABIN2668542)

Chung, Xu, Fuchs, Mund, Lange, Staege, Schubert, Bian, Dunkel, Eberharter, Regnard, Klinker, Meierhofer, Cozzuto, Winterpacht, Di Croce, Min, Will, Kinkley: "PHF13 is a molecular reader and transcriptional co-regulator of H3K4me2/3." dans: eLife, Vol. 5, (2016) (PubMed).

Harlen, Trotta, Smith, Mosaheb, Fuchs, Churchman: "Comprehensive RNA Polymerase II Interactomes Reveal Distinct and Varied Roles for Each Phospho-CTD Residue." dans: Cell reports, Vol. 15, Issue 10, pp. 2147-2158, (2016) (PubMed).

Takahashi, Takigawa, Watanabe, Anwar, Shibata, Tomomori-Sato, Sato, Ranjan, Seidel, Tsukiyama, Mizushima, Hayashi, Ohkawa, Conaway, Conaway, Hatakeyama: "MED26 regulates the transcription of snRNA genes through the recruitment of little elongation complex." dans: Nature communications, Vol. 6, pp. 5941, (2015) (PubMed).

Laitem, Zaborowska, Isa, Kufs, Dienstbier, Murphy: "CDK9 inhibitors define elongation checkpoints at both ends of RNA polymerase II-transcribed genes." dans: Nature structural & molecular biology, Vol. 22, Issue 5, pp. 396-403, (2015) (PubMed).

Hintermair, Heidemann, Koch, Descostes, Gut, Gut, Fenouil, Ferrier, Flatley, Kremmer, Chapman, Andrau, Eick: "Threonine-4 of mammalian RNA polymerase II CTD is targeted by Polo-like kinase 3 and required for transcriptional elongation." dans: The EMBO journal, Vol. 31, Issue 12, pp. 2784-97, (2012) (PubMed).

Chapman, Heidemann, Albert, Mailhammer, Flatley, Meisterernst, Kremmer, Eick: "Transcribing RNA polymerase II is phosphorylated at CTD residue serine-7." dans: Science (New York, N.Y.), Vol. 318, Issue 5857, pp. 1780-2, (2007) (PubMed).

Détails sur Rpb1 CTD

Antigène: Rpb1 CTD (RNA Polymerase II CTD)

Autre désignation: RNA Pol II CTD

Sujet: RNA pol II (RNA polymerase II) is responsible for synthesizing messenger RNA in eukaryotes. RNA pol II contains a carboxy terminal domain composed of heptapeptide repeats that are essential for polymerase activity. These repeats contain serine and threonine residues that are phosphorylated in actively transcribing RNA polymerase. In addition, RNA pol II, in combination with several other polymerase subunits, form the DNA binding domain of the polymerase, a groove in which the DNA template is transcribed into RNA. During the transcription cycle, the CTD of the large subunit of RNA pol II is reversibly phosphorylated. RNA pol II containing unphosphorylated CTD is recruited to the promoter, whereas the hyperphosphorylated CTD form is involved in active transcription. Phosphorylation occurs at two sites within the heptapeptide repeat, at serine 2, serine 5 and serine 7. RNA pol II Serine 7 phosphorylation is confined to promoter regions and is necessary for the initiation of transcription.

Poids moléculaire: 240 kDa

ID gène: 5430

NCBI Accession: NP_000928