MITF anticorps (N-Term)

N° du produit ABIN2668714

Il existe 3+ publications pour ce produit. L’anticorps anti-MITF Monoclonal Souris est utilisé pour la détection de MITF dans des échantillons de Humain. Il a été validé pour WB, IF, ICC, IP, ChIP-seq, ChIP et SSA.

Aperçu rapide pour MITF anticorps (N-Term) (ABIN2668714)

Antigène: MITF (Microphthalmia-Associated Transcription Factor (MITF))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp MITF est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), Immunoprecipitation (IP), ChIP DNA-Sequencing (ChIP-seq), Chromatin Immunoprecipitation (ChIP), Supershift Assay (SSA)

Clone: C5-D5

Détail du produit anti-MITF anticorps

Épitope: N-Term

Fonction: MITF antibody (mAb) (Clone C5.D5)

Purification: Protein G Chromatography

Immunogène: This MITF antibody was raised against an N-terminal fragment of human MITF.

Isotype: IgG1

Information d'application

Indications d'application: ChIP: 2.5 - 10 μg per ChIP ChIP-Seq: 4 μg each This antibody is also available as an AbFlex engineered recombinant antibody. For details on the corresponding AbFlex Recombinant Antibody, see Catalog No. 91201.

Restrictions: For Research Use only

Stockage

Concentration: 1 μg/μL

Buffer: Purified IgG in 70 mM Tris ( pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30 % glycerol and 0.035 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

Stock: -20 °C

Stockage commentaire: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Date de péremption: 24 months

Références pour anticorps anti-MITF (ABIN2668714)

Wellbrock, Marais: "Elevated expression of MITF counteracts B-RAF-stimulated melanocyte and melanoma cell proliferation." dans: The Journal of cell biology, Vol. 170, Issue 5, pp. 703-8, (2005) (PubMed).

Du, Miller, Widlund, Horstmann, Ramaswamy, Fisher: "MLANA/MART1 and SILV/PMEL17/GP100 are transcriptionally regulated by MITF in melanocytes and melanoma." dans: The American journal of pathology, Vol. 163, Issue 1, pp. 333-43, (2003) (PubMed).

Hemesath, Price, Takemoto, Badalian, Fisher: "MAP kinase links the transcription factor Microphthalmia to c-Kit signalling in melanocytes." dans: Nature, Vol. 391, Issue 6664, pp. 298-301, (1998) (PubMed).

Détails sur MITF

Antigène: MITF (Microphthalmia-Associated Transcription Factor (MITF))

Autre désignation: MITF

Sujet: MITF protein (Microphthalmia-associated Transcription Factor) is a basic helix-loop-helix type transcription factor involved in the differentiation and development of retinal pigment epithelium and melanocytes. It is also responsible for pigment cell-specific transcription of the melanogenesis enzyme genes tyrosinase and tyrosinase related protein 1. For activity MITF needs to heterodimerize with other bHLH proteins such as TFE3, TFEB or TFE. Defects in MITF are the cause of Waardenburg syndrome type 2A and Tietz syndrome.

Poids moléculaire: 52-56 kDa

ID gène: 4286

NCBI Accession: NP_937802

Pathways: Chromatin Binding