HDAC1 anticorps (AA 1-5, AA 433-448, AA 467-482)

N° du produit ABIN2668768

Il existe 1 publication pour ce produit. L’anticorps anti-HDAC1 Polyclonal Lapin est utilisé pour la détection de HDAC1 dans des échantillons de Humain, Souris et Rat. Il a été validé pour WB, ChIP et ChIP-seq.

Aperçu rapide pour HDAC1 anticorps (AA 1-5, AA 433-448, AA 467-482) (ABIN2668768)

Antigène: HDAC1 (Histone Deacetylase 1 (HDAC1))

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp HDAC1 est non-conjugé

Application: Western Blotting (WB), Chromatin Immunoprecipitation (ChIP), ChIP DNA-Sequencing (ChIP-seq)

Détail du produit anti-HDAC1 anticorps

Épitope: AA 1-5, AA 433-448, AA 467-482

Fonction: HDAC1 antibody (pAb)

Purification: Affinity Purified

Immunogène: This HDAC1 antibody was raised against a mixture of synthetic peptides corresponding to amino acid residues 1-5, 433-448 and 467-482 of human HDAC1.

Isotype: IgG

Information d'application

Indications d'application: Validated Applications: ChIP: 4 μg per ChIP ChIP-Seq: 4 μg each WB: 1 - 2 μg/mL dilution For optimal results in Western blotting, primary antibody incubations should be performed at room temperature. The addition of 0.1 % Tween 20 to all Blotto solutions may also reduce background. Individual optimization may be required.

Restrictions: For Research Use only

Stockage

Concentration: 0.5 μg/μL

Buffer: PBS containing 0.05 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

Stock: 4 °C,-20 °C

Stockage commentaire: Some products may be shipped at room temperature. This will not affect their stability or performance. Store at 4°C for short term. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Date de péremption: 24 months

Références pour anticorps anti-HDAC1 (ABIN2668768)

Xiong, Svingen, Sarmento, Smyrk, Dave, Khanna, Lomberk, Urrutia, Faubion: "Differential coupling of KLF10 to Sin3-HDAC and PCAF regulates the inducibility of the FOXP3 gene." dans: American journal of physiology. Regulatory, integrative and comparative physiology, Vol. 307, Issue 6, pp. R608-20, (2014) (PubMed).

Détails sur HDAC1

Antigène: HDAC1 (Histone Deacetylase 1 (HDAC1))

Autre désignation: HDAC1

Sujet: HDAC1 (Histone Deacetylase 1, also designated HD1) is a member of the class I mammalian histone deacetylases (HDACs) involved in regulating chromatin structure during transcription. These enzymes catalyze the removal of acetyl groups from lysine residues of histones and other cellular proteins. Lysine N-e-acetylation is a dynamic, reversible and tightly regulated protein and histone modification that plays a major role in regulation of gene expression in various cellular functions. It consists of the transfer of an acetyl moiety from an acetyl coenzyme A to the e-amino group of a lysine residue. In vivo, acetylation is controlled by the antagonistic activities of histone acetyltransferases (HATs) and histone deacetylases (HDACs). The HDACs are grouped into four classes, on the basis of similarity to yeast counterparts: class I (HDAC1, HDAC2, HDAC3 and HDAC8), class II (HDAC4, HDAC5, HDAC6, HDAC7, 9 and 10), class III (SIRT1-7) and class IV (HDAC11). HDAC1 and HDAC2 are recruited to Mad-Max complexes, which associate with the mSin3 scaffold protein, and are required for the transcriptional repression of Mad-Max target genes. HDAC1 is also involved in the regulation of p53. HDAC1 is expressed in various tissues. HDAC1, HDAC2 and HDAC3 are also ubiquitously expressed and can deacetylate both H3 and H4 in free histones or nucleosome substrate.

Poids moléculaire: 60 kDa

ID gène: 3065

NCBI Accession: NP_004955

Pathways: Neurotrophin Signaling Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Mitotic G1-G1/S Phases, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development, Negative Regulation of intrinsic apoptotic Signaling, Embryonic Body Morphogenesis