Caspase 3 anticorps (Biotin)
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- Antigène Voir toutes Caspase 3 (CASP3) Anticorps
- Caspase 3 (CASP3)
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Reactivité
- Humain, Souris
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Hôte
- Lapin
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Clonalité
- Monoclonal
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Conjugué
- Cet anticorp Caspase 3 est conjugé à/à la Biotin
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Application
- Intracellular Staining (ICS)
- Marque
- BD Pharmingen™
- Attributs du produit
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The caspase family of cysteine proteases plays a key role in apoptosis and inflammation. Caspase-3 is a key protease that is activated during the early stages of apoptosis and, like other members of the caspase family, is synthesized as an inactive pro-enzyme that is processed in cells undergoing apoptosis by self-proteolysis and/or cleavage by another protease. The processed forms of caspases consist of large (17-22 kDa) and small (10-12 kDa) subunits which associate to form an active enzyme. Active caspase-3, a marker for cells undergoing apoptosis, consists of a heterodimer of 17 and 12 kDa subunits which is derived from the 32 kDa pro-enzyme. Active caspase-3 proteolytically cleaves and activates other caspases, as well as relevant targets in the cytoplasm, e.g., D4-GDI and Bcl-2, and in the nucleus (e.g. PARP). This antibody has been reported to specifically recognize the active form of caspase-3 in human and mouse cells. It has not been reported to recognize the pro-enzyme form of caspase-3. Flow cytometric analysis of apoptotic and non-apoptotic populations for active caspase-3. Jurkat cells (Human T-cell leukemia, ATCC TIB-152) were left untreated (left panel) or treated with 4 μM of camptothecin for 4 hr to induce apoptosis (right panel). Cells were washed once in PBS, then fixed and permeabilized using the BD Cytofix/Cytoperm™ Kit (Cat. No. 554714) for 20 min at room temperature (RT), pelleted and washed with BD Perm/Wash™ buffer (component of Cat. No. 554714). Cells were subsequently stained with the biotin rabbit anti- active caspase-3 antibody (clone C92-605). Cells were then washed and resuspended in BD Perm/Wash™ buffer containing FITC-Avidin (5 ng/1x10e6 cells) [Cat. No. 554057] and incubated for 30 min at RT in the dark. Cells were then washed and resuspended in BD Perm/Wash™ buffer before analyzing by flow cytometry. The results show that untreated cells were primarily negative for active caspase-3 (left panel, M1), whereas more than half of the treated cells were positive for active caspase-3 staining (right panel, M2).
BD Pharmingen™ Biotin Rabbit Anti- Active Caspase-3 - Biotin - Clone C92-605 - Isotype Rabbit IgG - Reactivity Hu, Ms - 100 Tests - Purification
- The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
- Clone
- C92
- Isotype
- IgG
- Top Product
- Discover our top product CASP3 Anticorps primaire
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- Indications d'application
- Optimal working dilution should be determined by the investigator.
- Volume d'échantillon
- 20 μL
- Restrictions
- For Research Use only
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- Buffer
- Aqueous buffered solution containing BSA and ≤0.09 % sodium azide.
- Agent conservateur
- Sodium azide
- Précaution d'utilisation
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Conseil sur la manipulation
- The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed.
- Stock
- 4 °C
- Stockage commentaire
- Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
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Interferon gamma induces upregulation and activation of caspases 1, 3, and 8 to produce apoptosis in human erythroid progenitor cells." dans: Blood, Vol. 93, Issue 10, pp. 3309-16, (1999) (PubMed).
: "Involvement of Bcl-2 cleavage in the acceleration of VP-16-induced U937 cell apoptosis." dans: Biochemical and biophysical research communications, Vol. 246, Issue 2, pp. 484-8, (1998) (PubMed).
: "Caspases: enemies within." dans: Science (New York, N.Y.), Vol. 281, Issue 5381, pp. 1312-6, (1998) (PubMed).
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Interferon gamma induces upregulation and activation of caspases 1, 3, and 8 to produce apoptosis in human erythroid progenitor cells." dans: Blood, Vol. 93, Issue 10, pp. 3309-16, (1999) (PubMed).
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- Antigène
- Caspase 3 (CASP3)
- Autre désignation
- Caspase-3 (CASP3 Produits)
- Synonymes
- anticorps CPP32, anticorps CPP32B, anticorps SCA-1, anticorps A830040C14Rik, anticorps AC-3, anticorps Apopain, anticorps CC3, anticorps Caspase-3, anticorps Lice, anticorps Yama, anticorps mldy, anticorps xcpp32, anticorps casp3, anticorps zgc:100890, anticorps CASP-3, anticorps caspase-3, anticorps caspase 3, anticorps caspase 3 S homeolog, anticorps caspase 3, apoptosis-related cysteine peptidase a, anticorps caspase 3, apoptosis-related cysteine peptidase, anticorps CASP3, anticorps Casp3, anticorps casp3.S, anticorps casp3a
- Sujet
- Synonyms: CPP32, Yama, Apopain
- Pathways
- Apoptose, Caspase Cascade in Apoptosis, Sensory Perception of Sound, ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Activated T Cell Proliferation
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