L’anticorps Souris Monoclonal anti-LAMP1 a été validé pour ICS. Il convient pour détecter LAMP1 dans des échantillons de Humain. Il y a 2+ publications disponibles.
Reacts with the heavily glycosylated 110 kDa lysosomal-associated membrane protein, LAMP-1. LAMP-1 is a widely expressed intracellular antigen. It is also expressed on the surface of activated platelets, PHA-activated lymphocytes and some tumor cell lines, including U937 and KG1a. LAMP-1 has been shown to be a ligand for E-selectin-mediated cell adhesion. LAMP-1 and LAMP-2 (CD107b) are carriers for poly-N-acetyllactosamines, and are able to display sialyl Le[x] termini. This antibody is routinely tested by flow cytometric analysis. Other applications were tested during antibody development only or reported in the literature. Profile of permeabilized jurkat cells analyzed on a FACScan
Optimal working dilution should be determined by the investigator.
Volume d'échantillon
20 μL
Restrictions
For Research Use only
Buffer
Aqueous buffered solution containing BSA and ≤0.09 % sodium azide.
Agent conservateur
Sodium azide
Précaution d'utilisation
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Conseil sur la manipulation
The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed.
Stock
4 °C
Stockage commentaire
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Febbraio, Silverstein: "Identification and characterization of LAMP-1 as an activation-dependent platelet surface glycoprotein." dans: The Journal of biological chemistry, Vol. 265, Issue 30, pp. 18531-7, (1990) (PubMed).
Guesdon, Ternynck, Avrameas: "The use of avidin-biotin interaction in immunoenzymatic techniques." dans: The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, Vol. 27, Issue 8, pp. 1131-9, (1979) (PubMed).
Antigène
LAMP1
(Lysosomal-Associated Membrane Protein 1 (LAMP1))