ITGAL anticorps

N° du produit ABIN2688902

Cet anticorps Rat Monoclonal détecte spécifiquement ITGAL dans FACS, IHC (fro) et BR. Il présente une réactivité avec des échantillons de Souris. Il a été cité dans 6+ publications.

Aperçu rapide pour ITGAL anticorps (ABIN2688902)

Antigène: ITGAL (Integrin alpha-L (ITGAL))

Reactivité: Souris

Hôte: Rat

Clonalité: Monoclonal

Conjugué: Cet anticorp ITGAL est non-conjugé

Application: Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Blocking Reagent (BR)

Clone: 2D7

Détail du produit anti-ITGAL anticorps

Attributs du produit: The 2D7 antibody reacts with the 180- kDa αL chain of LFA-1 (CD11a/CD18, αLβ2 integrin), a heterodimeric surface glycoprotein expressed on almost all leukocytes. CD8a+CD8- intestinal intraepithelial T lymphocytes, which are believed to be thymus independent, do not express CD11a. LFA-1 mediates a variety of heterotypic and homotypic intracellular adhesions through interaction with ICAM-1 (CD54) and ICAM-2 (CD102), including participation in the immunological synapses between CD8+ T lymphocytes and antigen-presenting cells. mAb 2D7 has been reported to block an in vitro allogeneic mixed-leukocyte reaction. The 2D7 and M17/4 (Cat. No. 553337, for the NA/LE™ format) antibodies are reported to recognize different epitopes of the CD11a molecule. This antibody is routinely tested by flow cytometric analysis. Other applications were tested during antibody development only or reported in the literature.

BD Pharmingen™ Purified Rat Anti-Mouse CD11a - Purified - Clone 2D7 - Isotype Rat IgG2a, κ - Reactivity Ms - 0.5 mg

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogène: Not Reported

Isotype: IgG2a kappa

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Stockage

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Store undiluted at 4°C.

Références pour anticorps anti-ITGAL (ABIN2688902)

Potter, Grebe, Freiberg, Kupfer: "Formation of supramolecular activation clusters on fresh ex vivo CD8+ T cells after engagement of the T cell antigen receptor and CD8 by antigen-presenting cells." dans: Proceedings of the National Academy of Sciences of the United States of America, Vol. 98, Issue 22, pp. 12624-9, (2001) (PubMed).

Kootstra, Van Der Giezen, Van Krieken, De Heer, Bruijn: "Effective treatment of experimental lupus nephritis by combined administration of anti-CD11a and anti-CD54 antibodies." dans: Clinical and experimental immunology, Vol. 108, Issue 2, pp. 324-32, (1997) (PubMed).

Masten, Yates, Pollard Koga, Lipscomb: "Characterization of accessory molecules in murine lung dendritic cell function: roles for CD80, CD86, CD54, and CD40L." dans: American journal of respiratory cell and molecular biology, Vol. 16, Issue 3, pp. 335-42, (1997) (PubMed).

Huleatt, Lefrançois: "Beta2 integrins and ICAM-1 are involved in establishment of the intestinal mucosal T cell compartment." dans: Immunity, Vol. 5, Issue 3, pp. 263-73, (1996) (PubMed).

Springer: "Traffic signals for lymphocyte recirculation and leukocyte emigration: the multistep paradigm." dans: Cell, Vol. 76, Issue 2, pp. 301-14, (1994) (PubMed).

Larson, Springer: "Structure and function of leukocyte integrins." dans: Immunological reviews, Vol. 114, pp. 181-217, (1990) (PubMed).

Détails sur ITGAL

Antigène: ITGAL (Integrin alpha-L (ITGAL))

Autre désignation: CD11a

Sujet: Synonyms: Integrin αL chain, LFA-1α

Pathways: Activated T Cell Proliferation, Integrin Complex