Angiotensin I Converting Enzyme 1 anticorps

N° du produit ABIN2688976

Détail du produit anti-Angiotensin I Converting Enzyme 1 anticorps

Antigène: Angiotensin I Converting Enzyme 1 (ACE) (Angiotensin I Converting Enzyme (Peptidyl-Dipeptidase A) 1 (ACE))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Angiotensin I Converting Enzyme 1 est non-conjugé

Application: Flow Cytometry (FACS)

Marque: BD Pharmingen™

Attributs du produit: The BB9 Monoclonal antibody specifically binds Angiotensin-converting enzyme (ACE) which is also known as Dipeptidyl carboxypeptidase 1 (DCP1) or CD143. CD143 is a type I transmembrane glycoprotein that functions as a zinc metallopeptidase involved with the metabolism of angiotensin, bradykinin, and other oligopeptides. It is expressed on primitive hematopoietic progenitor cells (HPC). BB9 reacts with stromal cells, a minor subset of human bone marrow cells, it does not react with peripheral blood leucocytes. BB9 recognizes a subset of CD34+CD90+ cells that demonstrate low or absent expression of CD38, low retention of Rhodamine 123 and are Ki-67-negative. CD34+BB9+ cells are able to sustain hematopoiesis in pre-CFU culture stimulated with IL-3, IL-6, G-CSF, and SCF. BB9 also reacts with a subset of CD34+ cells from mobilized peripheral blood. This antibody was generated using human bone marrow-derived stromal cells as immunogen. Flow cytometric analysis of CD143 expression on human bone marrow cells. Human bone marrow cells were stained with APC Anti-Human CD34 (hematopoietic progenitor cell marker) and Purified Mouse Anti-Human Angiotensin Converting Enzyme (CD143) followed by FITC Goat Anti-Mouse IgG/IgM (Cat. No. 555988). The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of viable cells. Flow cytometric analysis of CD143 expression on human bone marrow cell line. HEL 92.1.7 (Human bone marrow erythroblast, ATCC TIB-180) cells were stained with either Purified Mouse IgG1, κ Isotype Control (Cat. No. 555746, Dashed line histogram) or Purified Mouse Anti-Human Angiotensin Converting Enzyme (CD143) (Solid line historgram), followed by FITC Goat Anti-Mouse IgG/IgM (Cat. No. 555988). The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of viable cells.

BD Pharmingen™ Purified Mouse Anti-Human Angiotensin Converting Enzyme (CD143) - Purified - Clone BB9 - Isotype Mouse IgG1, κ - Reactivity Hu - 0.1 mg

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogène: Human bone marrow-derived stromal cells

Clone: BB9

Isotype: IgG1 kappa

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Stockage

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Store undiluted at 4°C.

Références pour anticorps anti-Angiotensin I Converting Enzyme 1 (ABIN2688976)

Sinka, Biasch, Khazaal, Péault, Tavian: "Angiotensin-converting enzyme (CD143) specifies emerging lympho-hematopoietic progenitors in the human embryo." dans: Blood, Vol. 119, Issue 16, pp. 3712-23, (2012) (PubMed).

Zambidis, Park, Yu, Tam, Levine, Yuan, Pryzhkova, Péault: "Expression of angiotensin-converting enzyme (CD143) identifies and regulates primitive hemangioblasts derived from human pluripotent stem cells." dans: Blood, Vol. 112, Issue 9, pp. 3601-14, (2008) (PubMed).

Jokubaitis, Sinka, Driessen, Whitty, Haylock, Bertoncello, Smith, Péault, Tavian, Simmons: "Angiotensin-converting enzyme (CD143) marks hematopoietic stem cells in human embryonic, fetal, and adult hematopoietic tissues." dans: Blood, Vol. 111, Issue 8, pp. 4055-63, (2008) (PubMed).

Détails sur Angiotensin I Converting Enzyme 1

Antigène: Angiotensin I Converting Enzyme 1 (ACE) (Angiotensin I Converting Enzyme (Peptidyl-Dipeptidase A) 1 (ACE))

Autre désignation: CD143 (ACE Produits)

Synonymes: anticorps ACE1, anticorps CD143, anticorps DCP, anticorps DCP1, anticorps ICH, anticorps MVCD3, anticorps AW208573, anticorps Dcp1, anticorps StsRR92, anticorps dcp, anticorps ace1, anticorps dcp1, anticorps xace, anticorps cd143, anticorps ACE, anticorps angiotensin I converting enzyme, anticorps angiotensin I converting enzyme (peptidyl-dipeptidase A) 1, anticorps angiotensin-converting enzyme, anticorps angiotensin I converting enzyme (peptidyl-dipeptidase A) 3, anticorps angiotensin-converting enzyme-like, anticorps ACE, anticorps Ace, anticorps ace, anticorps CpipJ_CPIJ009106, anticorps ACE3, anticorps LOC101824864

Sujet: Synonyms: ACE1, DCP1, Kininase II

Pathways: ACE Inhibitor Pathway, Peptide Hormone Metabolism, Regulation of Systemic Arterial Blood Pressure by Hormones, Feeding Behaviour, Smooth Muscle Cell Migration