CD25 anticorps (Biotin)

N° du produit ABIN2689121

Cet anticorps Rat Monoclonal détecte spécifiquement CD25 dans FACS, IHC (fro) et IHC (p). Il présente une réactivité avec des échantillons de Souris. Il a été cité dans 6+ publications.

Aperçu rapide pour CD25 anticorps (Biotin) (ABIN2689121)

Antigène: CD25 (IL2RA) (Interleukin 2 Receptor, alpha (IL2RA))

Reactivité: Souris

Hôte: Rat

Clonalité: Monoclonal

Conjugué: Cet anticorp CD25 est conjugé à/à la Biotin

Application: Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: 7D4

Détail du produit anti-CD25 anticorps (Biotin)

Attributs du produit: The 7D4 antibody reacts with CD25, the low affinity IL-2 Receptor (IL-2Rα, p55) expressed on activated T and B lymphocytes from all mouse strains tested. IL-2Rα by itself is not a signaling receptor. However, it can combine with IL-2 Receptor β (CD122) and γ (CD132) chains to form high-affinity, signaling receptor complexes for IL-2. Resting T and B lymphocytes and resting and activated NK cells do not express IL-2Rα. CD25 is transiently expressed at a low level during normal B-cell development in the bone marrow on the CD45R/B220low TdT- sIg- Pre-B/Pre-B-II and CD45R/B220low TdT- sIgM+ sIgD- immature B stages, but not on the CD45R/B220low TdT+ sIg- Pro-B/Pre-B-I stage nor on CD45R/B220high TdTsIgM+ sIgD+ mature B cells. It is expressed at a higher level during a very early stage of T-cell development in fetal and adult thymus. Peripheral CD25+ CD4+ T lymphocytes called regulatory T (Treg) cells are involved in the maintenance of self-tolerance. It has also been reported that dendritic cells express CD25, recognized by mAb 7D4 (Cat. no. 553068). The 3C7 antibody recognizes an epitope of CD25 which is distinct from those recognized by mAbs 7D4 and PC61 (Cat. no. 553866), and it blocks binding of IL-2 to CD25. Immunohistochemical staining of CD25 positive cells. Frozen sections of mouse thymus were reacted with the anti-CD25 antibody. Immature thymocytes expressing the IL-2 receptor can be identified by the brown labeling of their cell membranes. Amplification 20x.

BD Pharmingen™ Biotin Rat Anti-Mouse CD25 - Biotin - Clone 7D4 - Isotype Rat IgM, κ - Reactivity Ms - 1 mL

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogène: IL-2-dependent BALB/c mouse helper T-cell clone HT-2

Isotype: IgM kappa

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 125 μg/mL

Buffer: Aqueous buffered solution containing BSA, goat serum, and ≤0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed.

Stock: 4 °C

Stockage commentaire: Store undiluted at 4°C.

Références pour anticorps anti-CD25 (Biotin) (ABIN2689121)

Chen, Ma, Young, Alt: "IL-2 receptor alpha chain expression during early B lymphocyte differentiation." dans: International immunology, Vol. 6, Issue 8, pp. 1265-8, (1995) (PubMed).

Taniguchi, Minami: "The IL-2/IL-2 receptor system: a current overview." dans: Cell, Vol. 73, Issue 1, pp. 5-8, (1993) (PubMed).

Garni-Wagner, Witte, Tutt, Kuziel, Tucker, Bennett, Kumar: "Natural killer cells in the thymus. Studies in mice with severe combined immune deficiency." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 144, Issue 3, pp. 796-803, (1990) (PubMed).

Crowley, Inaba, Witmer-Pack, Steinman: "The cell surface of mouse dendritic cells: FACS analyses of dendritic cells from different tissues including thymus." dans: Cellular immunology, Vol. 118, Issue 1, pp. 108-25, (1989) (PubMed).

Habu, Okumura, Diamantstein, Shevach: "Expression of interleukin 2 receptor on murine fetal thymocytes." dans: European journal of immunology, Vol. 15, Issue 5, pp. 456-60, (1985) (PubMed).

Malek, Schmidt, Shevach: "The murine IL 2 receptor. III. Cellular requirements for the induction of IL 2 receptor expression on T cell subpopulations." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 134, Issue 4, pp. 2405-13, (1985) (PubMed).

Détails sur CD25

Antigène: CD25 (IL2RA) (Interleukin 2 Receptor, alpha (IL2RA))

Autre désignation: CD25

Sujet: Synonyms: IL-2 Receptor α chain, p55

Pathways: Signalistation JAK/STAT, Growth Factor Binding, Activated T Cell Proliferation