CD3 anticorps

N° du produit ABIN2689163

Cet anticorps Souris Monoclonal détecte spécifiquement CD3 dans FACS, IP, IHC (fro), CyTox et FM. Il présente une réactivité avec des échantillons de Rat. Il a été cité dans 4+ publications.

Aperçu rapide pour CD3 anticorps (ABIN2689163)

Antigène: CD3

Reactivité: Rat

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CD3 est non-conjugé

Application: Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro)), Cytotoxicity Test (CyTox), Fluorescence Microscopy (FM)

Clone: 1F4

Détail du produit anti-CD3 anticorps

Attributs du produit: The 1F4 antibody reacts with the T-cell receptor-associated CD3 cell-surface antigen found on thymocytes, peripheral T lymphocytes, and dendritic epidermal T cells. In vitro, immobilized 1F4 mAb induces T-cell proliferation, and soluble mAb synergizes with PMA for T-cell activation. Pre-incubation of splenocytes with mAb 1F4 blocks staining with mAb G4.18 (Cat. No. 559974/554830). This antibody is routinely tested by flow cytometric analysis. Other applications were tested during antibody development only or reported in the literature. CD3e expression in spleen and thymus. LEW splenocytes were preincubated with Rat BD Fc Block™ purified anti-rat CD32 mAb D34-485 (Cat. No. 550270/550271) and simultaneously stained with FITC-onjugated anti-rat CD5 mAb OX-19 (Cat. No. 551449, left panels) and purified mAb 1F4 (middle left panel), followed by PE-conjugated anti-mouse IgMa mAb DS-1 (Cat. No. 553517, left panels). LEW thymocytes were stained with purified 1F4 mAb (far right panel) followed by PE-conjugated anti-mouse IgMa mAb DS-1 (right panels). Flow cytometry was performed on a BD FACSCalibur™ flow cytometry system.

BD Pharmingen™ Purified Mouse Anti-Rat CD3 - Purified - Clone 1F4 - Isotype Mouse IgM, κ - Reactivity Rat - 0.5 mg

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogène: F344 rat splenic T cells stimulated with PMA plus A23187

Isotype: IgM kappa

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Stockage

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Store undiluted at 4°C.

Références pour anticorps anti-CD3 (ABIN2689163)

Naper, Vaage, Lambracht, Løvik, Butcher, Wonigeit, Rolstad: "Alloreactive natural killer cells in the rat: complex genetics of major histocompatibility complex control." dans: European journal of immunology, Vol. 25, Issue 5, pp. 1249-56, (1995) (PubMed).

Elbe, Kilgus, Hünig, Stingl: "T-cell receptor diversity in dendritic epidermal T cells in the rat." dans: The Journal of investigative dermatology, Vol. 102, Issue 1, pp. 74-9, (1994) (PubMed).

Nicolls, Aversa, Pearce, Spinelli, Berger, Gurley, Hall: "Induction of long-term specific tolerance to allografts in rats by therapy with an anti-CD3-like monoclonal antibody." dans: Transplantation, Vol. 55, Issue 3, pp. 459-68, (1993) (PubMed).

Tanaka, Masuko, Yagita, Tamura, Hashimoto: "Characterization of a CD3-like rat T cell surface antigen recognized by a monoclonal antibody." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 142, Issue 8, pp. 2791-5, (1989) (PubMed).

Détails sur CD3

Antigène: CD3

Autre désignation: CD3

Pathways: TCR Signaling, Ubiquitin Proteasome Pathway