CD4 anticorps

N° du produit ABIN2689228

Cité dans 9 publications. Cet anticorps anti-CD4 Monoclonal Souris (Clone OX) (ABIN2689228) détecte spécifiquement CD4 dans FACS, IP, IHC (fro) et BR. L’anticorps est réactif avec des échantillons de Rat.

Aperçu rapide pour CD4 anticorps (ABIN2689228)

Antigène: CD4

Reactivité: Rat

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CD4 est non-conjugé

Application: Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro)), Blocking Reagent (BR)

Clone: OX

Détail du produit anti-CD4 anticorps

Attributs du produit: The OX-38 antibody has been reported to react with the CD4 antigen on most thymocytes, a subpopulation of mature T lymphocytes (i.e., MHC class II-restricted T cells, including most T helper cells), monocytes, macrophages, and some dendritic cells. CD4 is an antigen coreceptor on the T-cell surface which interacts with MHC class II molecules on antigen-presenting cells. It participates in T-cell activation through its association with the T-cell receptor complex and protein tyrosine kinase lck. The OX-38 antibody has been reported to bind to the same epitope of CD4 as that recognized by W3/25 mAb, which is a different epitope than that recognized by OX-35 mAb (Cat. No. 554837). In vivo blocking of some cell-mediated immune responses by mAb OX-38 has been reported. Injection of OX-38 mAb induces allograft unresponsiveness in rats, with varying results depending on the rat strain used (high or low responder). Furthermore, in vivo depletion of CD4+ lymphocytes has been reported with this antibody. This antibody is routinely tested by flow cytometric analysis. Other applications were tested during antibody development only or reported in the literature. Two-color analysis of the expression of CD4 on rat splenocytes. Lewis splenocytes were incubated simultaneously with PE-conjugated anti-rat CD3 G4.18 (Cat. No. 554833) and purified OX-38 (right panel), followed by FITC anti-mouse IgG2a R19-15 (Cat. No. 553390) monoclonal antibodies. The CD3-negative CD4-dim cells are the monocyte/macrophage population. Flow cytometry was performed on a BD FACScan™ flow cytometry system.

BD Pharmingen™ Purified Mouse Anti-Rat CD4 - Purified - Clone OX-38 - Isotype Mouse IgG2a, κ - Reactivity Rat - 0.5 mg

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogène: Rat thymocyte glycoproteins

Isotype: IgG2a kappa

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Stockage

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Store undiluted at 4°C.

Références pour anticorps anti-CD4 (ABIN2689228)

Liu, Zhang, Jenkins, MacPherson: "Dendritic cell heterogeneity in vivo: two functionally different dendritic cell populations in rat intestinal lymph can be distinguished by CD4 expression." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 161, Issue 3, pp. 1146-55, (1998) (PubMed).

Suzuki, Hara, Miyahara, Tanigawa, Matsuzaki, Onitsuka, Shibata, Koga, Yamashita, Miyamoto, Suzuki, Amemiya, Iwaya, Miyasaka, Kimura: "Microchimerism and graft acceptance: IV. Cardiac allograft acceptance following anti-adhesion molecule antibody therapy." dans: Transplantation proceedings, Vol. 28, Issue 4, pp. 2058-60, (1996) (PubMed).

Yin, Fathman: "Tissue-specific effects of anti-CD4 therapy in induction of allograft unresponsiveness in high and low responder rats." dans: Transplant immunology, Vol. 3, Issue 3, pp. 258-64, (1996) (PubMed).

Arima, Goss, Walp, Flye: "Administration of anti-CD4 monoclonal antibody with intrathymic injection of alloantigen results in rat cardiac allograft tolerance." dans: Surgery, Vol. 118, Issue 2, pp. 265-72; discussion 272-3, (1995) (PubMed).

Bañuls, Alvarez, Ferrero, Zapata, Ardavin: "Cell-surface marker analysis of rat thymic dendritic cells." dans: Immunology, Vol. 79, Issue 2, pp. 298-304, (1993) (PubMed).

Janeway: "The T cell receptor as a multicomponent signalling machine: CD4/CD8 coreceptors and CD45 in T cell activation." dans: Annual review of immunology, Vol. 10, pp. 645-74, (1992) (PubMed).

Stitz, Sobbe, Bilzer: "Preventive effects of early anti-CD4 or anti-CD8 treatment on Borna disease in rats." dans: Journal of virology, Vol. 66, Issue 6, pp. 3316-23, (1992) (PubMed).

Bierer, Sleckman, Ratnofsky, Burakoff: "The biologic roles of CD2, CD4, and CD8 in T-cell activation." dans: Annual review of immunology, Vol. 7, pp. 579-99, (1989) (PubMed).

Jefferies, Green, Williams: "Authentic T helper CD4 (W3/25) antigen on rat peritoneal macrophages." dans: The Journal of experimental medicine, Vol. 162, Issue 1, pp. 117-27, (1985) (PubMed).

Détails sur CD4

Antigène: CD4

Autre désignation: CD4

Pathways: TCR Signaling, Maintenance of Protein Location, CXCR4-mediated Signaling Events