CD4 anticorps (Biotin)

N° du produit ABIN2689240

Cet anticorps anti-CD4 est un anticorps Rat Monoclonal détectant CD4 dans FACS et IHC (fro). Adapté pour Souris. Ce Primary Antibody a été cité dans 7+ publications.

Aperçu rapide pour CD4 anticorps (Biotin) (ABIN2689240)

Antigène: CD4

Reactivité: Souris

Hôte: Rat

Clonalité: Monoclonal

Conjugué: Cet anticorp CD4 est conjugé à/à la Biotin

Application: Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: GK1-5

Détail du produit anti-CD4 anticorps (Biotin)

Marque: BD Pharmingen™

Attributs du produit: The GK1.5 clone has been reported to react with the CD4 (L3T4) differentiation antigen expressed on most thymocytes, a subpopulation of mature T lymphocytes (i.e., MHC class II-restricted T cells, including most T helper cells), and a subset of NK-T cells. In addition, CD4 has also been reported to be detectable on pluripotent hematopoietic stem cells, bone marrow myeliod and B-lymphocyte precursors, intrathymic lymphoid precursors, and a subset of splenic dendritic cells. CD4 has also been reported to be expressed on the plasma membrane of mouse egg cells and is involved in adhesion of the egg to MHC class II-bearing sperm. CD4 is an antigen coreceptor on the T-cell surface which interacts with MHC class II molecules on antigen-presenting cells. It participates in T-cell activation through its association with the T-cell receptor complex and protein tyrosine kinase lck. GK1.5 mAb reportedly blocks binding of the RM4-5 (Cat. No. 553046/553047) and H129.19 (Cat. No. 553650/553651), but not RM4-4 (Cat. No. 553055) antibodies. This antibody is routinely tested by flow cytometric analysis. Other applications were tested during antibody development only or reported in the literature.

BD Pharmingen™ Biotin Rat Anti-Mouse CD4 - Biotin - Clone GK1.5 - Isotype Rat IgG2b, κ - Reactivity Ms - 0.5 mg

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogène: Mouse CTL clone V4

Isotype: IgG2b kappa

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Stockage

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed.

Stock: 4 °C

Stockage commentaire: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

Références pour anticorps anti-CD4 (Biotin) (ABIN2689240)

Guo, Watanabe, Mori, Mori: "Molecular structure and function of CD4 on murine egg plasma membrane." dans: Zygote (Cambridge, England), Vol. 3, Issue 1, pp. 65-73, (1995) (PubMed).

Baron, Reich, Visintin, Janeway: "The pathogenesis of adoptive murine autoimmune diabetes requires an interaction between alpha 4-integrins and vascular cell adhesion molecule-1." dans: The Journal of clinical investigation, Vol. 93, Issue 4, pp. 1700-8, (1994) (PubMed).

Janeway: "The T cell receptor as a multicomponent signalling machine: CD4/CD8 coreceptors and CD45 in T cell activation." dans: Annual review of immunology, Vol. 10, pp. 645-74, (1992) (PubMed).

Wineman, Gilmore, Gritzmacher, Torbett, Müller-Sieburg: "CD4 is expressed on murine pluripotent hematopoietic stem cells." dans: Blood, Vol. 80, Issue 7, pp. 1717-24, (1992) (PubMed).

Frederickson, Basch: "L3T4 antigen expression by hemopoietic precursor cells." dans: The Journal of experimental medicine, Vol. 169, Issue 4, pp. 1473-8, (1989) (PubMed).

Ghobrial, Boublik, Winn, Auchincloss: "In vivo use of monoclonal antibodies against murine T cell antigens." dans: Clinical immunology and immunopathology, Vol. 52, Issue 3, pp. 486-506, (1989) (PubMed).

Dialynas, Quan, Wall, Pierres, Quintáns, Loken, Pierres, Fitch: "Characterization of the murine T cell surface molecule, designated L3T4, identified by monoclonal antibody GK1.5: similarity of L3T4 to the human Leu-3/T4 molecule." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 131, Issue 5, pp. 2445-51, (1983) (PubMed).

Détails sur CD4

Antigène: CD4

Autre désignation: CD4

Sujet: Synonyms: L3T4

Pathways: TCR Signaling, Maintenance of Protein Location, CXCR4-mediated Signaling Events