CD44 anticorps

N° du produit ABIN2689261

Détail du produit anti-CD44 anticorps

Antigène: CD44

Reactivité: Souris

Hôte: Rat

Clonalité: Monoclonal

Conjugué: Cet anticorp CD44 est non-conjugé

Application: Flow Cytometry (FACS), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro)), Blocking Reagent (BR), Cytotoxicity Test (CyTox)

Marque: BD Pharmingen™

Attributs du produit: The IM7 antibody reacts with an epitope on both alloantigens and all isoforms of the CD44 glycoprotein (Pgp-1, Ly-24). The standard form of CD44, lacking variable exons and referred to as CD44H or CD44s, is widely expressed on hematopoietic and non-hematopoietic cells. CD44 isoforms encoded by variable exons are expressed on epithelial cells, but only at low levels on most leukocytes. Mice with the Ly-24.1 alloantigen (e.g., BALB/c, CBA/J, DBA/1, DBA/2) have relatively large subsets of CD44H+ T lymphocytes, while Ly-24.2 strains (e.g., A, AKR, CBA/N, C3H/He, C57BL, C57BR, C57L, C58, NZB, SJL, SWR, 129) have few CD44H+ T cells. CD44 is a cell adhesion receptor, and its principal ligand, hyaluronate, is a common component of extracellular matrices. Differential glycosylation of CD44 influences its binding to hyaluronate. Additional ligands include the cell-surface form of CD74 and the cytokine osteopontin (Eta-1). Bone marrow- and thymus-derived progenitor cells capable of repopulating the thymus express CD44. In the periphery, the level of CD44 expression increases upon activation of B lymphocytes, CD4+ T cells, and CD8+ T cells, memory cells can be recognized by their CD44[hi] phenotype. The IM7 mAb inhibits established collagen-induced arthritis in DBA/1 mice. Moreover, it prevents CNS inflammation and clinical symptoms of experimental autoimmune encephalomyelitis. In contrast, the same antibody exacerbates experimental autoimmune thyroiditis in CBA/J mice. The IM7 mAb recognizes a different epitope from that recognized by mAb KM114 (Cat. No. 558739), and the antibody pair can be used in ELISA to detect soluble CD44. It has been observed that IM7 antibody cross-reacts with human, dog, cat, horse, cow, and pig leukocytes. Anti-human CD44, clone G44-26 (Cat. No. 555476), and IM7 antibody compete for binding to human peripheral blood lymphocytes. This antibody is routinely tested by flow cytometric analysis. Other applications were tested during antibody development only or reported in the literature.

BD Pharmingen™ Purified Rat Anti-Mouse CD44 - Purified - Clone IM7 - Isotype Rat IgG2b, κ - Reactivity Ms - 0.5 mg

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogène: Dexamethasone-induced cells of the SJL mouse spontaneous myeloid leukemia M1

Clone: IM7

Isotype: IgG2b kappa

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Stockage

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Store undiluted at 4°C.

Références pour anticorps anti-CD44 (ABIN2689261)

Ikawa, Kawamoto, Fujimoto, Katsura: "Commitment of common T/Natural killer (NK) progenitors to unipotent T and NK progenitors in the murine fetal thymus revealed by a single progenitor assay." dans: The Journal of experimental medicine, Vol. 190, Issue 11, pp. 1617-26, (2000) (PubMed).

Parish, Brennan, Cooke: "Anti-CD44 treatment does not prevent the extravasation of autopathogenic T cells to the thyroid in experimental autoimmune thyroiditis." dans: Immunology, Vol. 97, Issue 3, pp. 533-9, (2000) (PubMed).

Brocke, Piercy, Steinman, Weissman, Veromaa: "Antibodies to CD44 and integrin alpha4, but not L-selectin, prevent central nervous system inflammation and experimental encephalomyelitis by blocking secondary leukocyte recruitment." dans: Proceedings of the National Academy of Sciences of the United States of America, Vol. 96, Issue 12, pp. 6896-901, (1999) (PubMed).

Nedvetzki, Walmsley, Alpert, Williams, Feldmann, Naor: "CD44 involvement in experimental collagen-induced arthritis (CIA)." dans: Journal of autoimmunity, Vol. 13, Issue 1, pp. 39-47, (1999) (PubMed).

Matsumoto, Nghiem, Nozaki, Schmits, Penninger: "Cooperation between CD44 and LFA-1/CD11a adhesion receptors in lymphokine-activated killer cell cytotoxicity." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 160, Issue 12, pp. 5781-9, (1998) (PubMed).

Naor, Sionov, Ish-Shalom: "CD44: structure, function, and association with the malignant process." dans: Advances in cancer research, Vol. 71, Issue 4, pp. 241-319, (1997) (PubMed).

Weiss, Sleeman, Renkl, Dittmar, Termeer, Taxis, Howells, Hofmann, Köhler, Schöpf, Ponta, Herrlich, Simon: "An essential role for CD44 variant isoforms in epidermal Langerhans cell and blood dendritic cell function." dans: The Journal of cell biology, Vol. 137, Issue 5, pp. 1137-47, (1997) (PubMed).

Weber, Ashkar, Glimcher, Cantor: "Receptor-ligand interaction between CD44 and osteopontin (Eta-1)." dans: Science (New York, N.Y.), Vol. 271, Issue 5248, pp. 509-12, (1996) (PubMed).

Bendelac: "Mouse NK1+ T cells." dans: Current opinion in immunology, Vol. 7, Issue 3, pp. 367-74, (1995) (PubMed).

Katoh, Zheng, Oritani, Shimozato, Kincade: "Glycosylation of CD44 negatively regulates its recognition of hyaluronan." dans: The Journal of experimental medicine, Vol. 182, Issue 2, pp. 419-29, (1995) (PubMed).

Hathcock, Hirano, Murakami, Hodes: "CD44 expression on activated B cells. Differential capacity for CD44-dependent binding to hyaluronic acid." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 151, Issue 12, pp. 6712-22, (1994) (PubMed).

Katoh, McCarthy, Kincade: "Characterization of soluble CD44 in the circulation of mice. Levels are affected by immune activity and tumor growth." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 153, Issue 8, pp. 3440-9, (1994) (PubMed).

Camp, Scheynius, Johansson, Puré: "CD44 is necessary for optimal contact allergic responses but is not required for normal leukocyte extravasation." dans: The Journal of experimental medicine, Vol. 178, Issue 2, pp. 497-507, (1993) (PubMed).

Lesley, Hyman, Kincade: "CD44 and its interaction with extracellular matrix." dans: Advances in immunology, Vol. 54, Issue 4, pp. 271-335, (1993) (PubMed).

Naujokas, Morin, Anderson, Peterson, Miller: "The chondroitin sulfate form of invariant chain can enhance stimulation of T cell responses through interaction with CD44." dans: Cell, Vol. 74, Issue 2, pp. 257-68, (1993) (PubMed).

Vremec, Zorbas, Scollay, Saunders, Ardavin, Wu, Shortman: "The surface phenotype of dendritic cells purified from mouse thymus and spleen: investigation of the CD8 expression by a subpopulation of dendritic cells." dans: The Journal of experimental medicine, Vol. 176, Issue 1, pp. 47-58, (1992) (PubMed).

MacDonald, Budd, Cerottini: "Pgp-1 (Ly 24) as a marker of murine memory T lymphocytes." dans: Current topics in microbiology and immunology, Vol. 159, Issue 4, pp. 97-109, (1990) (PubMed).

Lynch, Ceredig: "Mouse strain variation in Ly-24 (Pgp-1) expression by peripheral T cells and thymocytes: implications for T cell differentiation." dans: European journal of immunology, Vol. 19, Issue 2, pp. 223-9, (1989) (PubMed).

Spangrude, Klein, Heimfeld, Aihara, Weissman: "Two monoclonal antibodies identify thymic-repopulating cells in mouse bone marrow." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 142, Issue 2, pp. 425-30, (1989) (PubMed).

Budd, Cerottini, Horvath, Bron, Pedrazzini, Howe, MacDonald: "Distinction of virgin and memory T lymphocytes. Stable acquisition of the Pgp-1 glycoprotein concomitant with antigenic stimulation." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 138, Issue 10, pp. 3120-9, (1987) (PubMed).

Détails sur CD44

Antigène: CD44

Autre désignation: CD44 (CD44 Produits)

Synonymes: anticorps CDW44, anticorps CSPG8, anticorps ECMR-III, anticorps HCELL, anticorps HUTCH-I, anticorps IN, anticorps LHR, anticorps MC56, anticorps MDU2, anticorps MDU3, anticorps MIC4, anticorps Pgp1, anticorps AU023126, anticorps AW121933, anticorps AW146109, anticorps HERMES, anticorps Ly-24, anticorps Pgp-1, anticorps CD44A, anticorps METAA, anticorps RHAMM, anticorps XCD44, anticorps cd44, anticorps cdw44, anticorps cspg8, anticorps ecmr-iii, anticorps hcell, anticorps lhr, anticorps mc56, anticorps mdu2, anticorps mdu3, anticorps mic4, anticorps mutch-i, anticorps pgp1, anticorps CD44 molecule (Indian blood group), anticorps syndecan 4, anticorps CD44 antigen, anticorps CD44 molecule (Indian blood group) S homeolog, anticorps CD44, anticorps SDC4, anticorps Cd44, anticorps cd44.S

Sujet: Synonyms: Pgp-1, H-CAM, Ly-24

Pathways: Glycosaminoglycan Metabolic Process, Autophagy, Negative Regulation of intrinsic apoptotic Signaling