ITGA2 anticorps (Biotin)

N° du produit ABIN2689298

Détail du produit anti-ITGA2 anticorps (Biotin)

Antigène: ITGA2 (Integrin alpha 2 (ITGA2))

Reactivité: Souris

Hôte: Rat

Clonalité: Monoclonal

Conjugué: Cet anticorp ITGA2 est conjugé à/à la Biotin

Application: Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Marque: BD Pharmingen™

Attributs du produit: The rat anti-mouse CD49b monoclonal antibody (clone DX5) specifically binds to the integrin α2 chain (CD49b). CD49b is a 150 kDa transmembrane glycoprotein that non-covalently associates with CD29 (integrin β1) to form the integrin α2β1 complex known as VLA-2. The rat anti-mouse CD49b antibody (clone DX5) has been reported to identify the majority of NK cells and a small T-cell subpopulation in most mouse strains (e.g., A/J, AKR, BALB/c, C3H/HeJ, C57BL/6, C57BL/10, C57BR, C58, CBA/Ca, DBA/1, DBA/2, SJL, SWR, 129/J, but not NOD). The DX5 antibody also recognizes platelets that express high levels of CD49b. Multiparameter flow cytometric analysis has demonstrated that most lymphocytes which express NK-1.1 (NKR-P1B and NKR-P1C), as detectable by mouse anti-mouse NK-1.1 antibody (clone PK136), also express the DX5 antigen. Small DX5+ NK-1.1- and DX5- NK-1.1+ cell subsets are found, especially among the CD3-positive cell population. Some CD49b+ NK cells have been reported to gradually lose reactivity with the rat anti-mouse CD49b antibody (clone DX5) when cultured in the presence of recombinant human IL-2. The resulting DX5-negative cells have weakened cytotoxic activity when compared to the remaining DX5+ cells. This indicates that the DX5 antibody distinguishes functional subsets of NK cells. No activation or blocking activity of the rat anti-mouse antibody (clone DX5) has been observed. Staining of splenic NK cells with this antibody reportedly can be blocked by hamster anti-mouse CD49b antibody (clone HMα2). Detection of NK cells with monoclonal antibodies. Freshly isolated splenocytes from a C57BL/6 mouse were simultaneously stained with FITC Mouse anti-Mouse NK-1.1 antibody (Cat. No. 553164, both panels) and Biotin Rat anti-Mouse CD49b antibody (clone DX5) (right panel) followed by PE-Streptavidin (Cat. No. 554061, both panels). Flow cytometry was performed on a BD FACScan™ instrument .

BD Pharmingen™ Biotin Rat Anti-Mouse CD49b - Biotin - Clone DX5 - Isotype Rat IgM, κ - Reactivity Ms - 0.5 mg

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogène: Mouse (C57BL/6) NK1.1+ cells propagated with rIL-2

Clone: DX5

Isotype: IgM kappa

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Stockage

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed.

Stock: 4 °C

Stockage commentaire: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

Références pour anticorps anti-ITGA2 (Biotin) (ABIN2689298)

Arase, Saito, Phillips, Lanier: "Cutting edge: the mouse NK cell-associated antigen recognized by DX5 monoclonal antibody is CD49b (alpha 2 integrin, very late antigen-2)." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 167, Issue 3, pp. 1141-4, (2001) (PubMed).

Ortaldo, Winkler-Pickett, Mason, Mason: "The Ly-49 family: regulation of cytotoxicity and cytokine production in murine CD3+ cells." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 160, Issue 3, pp. 1158-65, (1998) (PubMed).

Moore, von Freeden-Jeffry, Murray, Zlotnik: "Inhibition of gamma delta T cell development and early thymocyte maturation in IL-7 -/- mice." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 157, Issue 6, pp. 2366-73, (1996) (PubMed).

Détails sur ITGA2

Antigène: ITGA2 (Integrin alpha 2 (ITGA2))

Autre désignation: CD49b (ITGA2 Produits)

Synonymes: anticorps BR, anticorps CD49B, anticorps GPIa, anticorps HPA-5, anticorps VLA-2, anticorps VLAA2, anticorps DX5, anticorps integrin subunit alpha 2, anticorps integrin alpha 2, anticorps ITGA2, anticorps Itga2

Sujet: Synonyms: itga2, Integrin alpha-2, DX5, Pan NK cell marker, VLAA2, VLA-2 alpha chain

Pathways: CXCR4-mediated Signaling Events, Smooth Muscle Cell Migration, Integrin Complex