CD80 anticorps (Biotin)

N° du produit ABIN2689412

Cet anticorps Souris Monoclonal détecte spécifiquement CD80 dans FACS. Il présente une réactivité envers Rat et a été mentionné dans 3+ publications.

Aperçu rapide pour CD80 anticorps (Biotin) (ABIN2689412)

Antigène: CD80

Reactivité: Rat

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CD80 est conjugé à/à la Biotin

Application: Flow Cytometry (FACS)

Clone: 3H5

Détail du produit anti-CD80 anticorps (Biotin)

Marque: BD Pharmingen™

Attributs du produit: The 3H5 antibody reacts with CD80 (B7-1), a member of the Ig superfamily of transmembrane proteins. CD80, a ligand for CD28 and CD152 (CTLA-4), is one of the accessory molecules that plays an important role in T cell-B cell costimulatory interactions. CD80 is predominantly expressed on antigen-presenting cells. It can be induced on splenic B cells by in vitro polyclonal activation such as LPS treatment. 3H5 mAb is reported to block the costimulatory function of rat CD80 and to immunoprecipitate CD80 from Lewis-S1 cell lysates. CD80 expression on spleen cells. 72-hour LPS-stimulated LOU splenocytes were simultaneously stained with FITC-conjugated anti-rat CD45RA mAb OX-33 (Cat. no. 554883) and biotinylated mAb 3H5, followed by Streptavidin-PE (Cat. no. 554061). Viable lymphocyte blasts are represented. Flow cytometry was performed on a BD FACScan™ flow cytometry system.

BD Pharmingen™ Biotin Mouse Anti-Rat CD80 - Biotin - Clone 3H5 - Isotype Mouse IgG1, κ - Reactivity Rat - 0.5 mg

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogène: Rat HTLV-1-transformed T-cell line Lewis-S1

Isotype: IgG1 kappa

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Stockage

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed.

Stock: 4 °C

Stockage commentaire: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

Références pour anticorps anti-CD80 (Biotin) (ABIN2689412)

Damoiseaux, Yagita, Okumura, van Breda Vriesman: "Costimulatory molecules CD80 and CD86 in the rat; tissue distribution and expression by antigen-presenting cells." dans: Journal of leukocyte biology, Vol. 64, Issue 6, pp. 803-9, (1998) (PubMed).

Maeda, Sato, Azuma, Yagita, Okumura: "Characterization of rat CD80 and CD86 by molecular cloning and mAb." dans: International immunology, Vol. 9, Issue 7, pp. 993-1000, (1997) (PubMed).

Bluestone: "New perspectives of CD28-B7-mediated T cell costimulation." dans: Immunity, Vol. 2, Issue 6, pp. 555-9, (1995) (PubMed).

Détails sur CD80

Antigène: CD80

Autre désignation: CD80

Sujet: Synonyms: B7-1

Pathways: TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Positive Regulation of Immune Effector Process, Cancer Immune Checkpoints