CD86 anticorps (Biotin)

N° du produit ABIN2689422

Détail du produit anti-CD86 anticorps (Biotin)

Antigène: CD86

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CD86 est conjugé à/à la Biotin

Application: Flow Cytometry (FACS)

Marque: BD Pharmingen™

Attributs du produit: IT2.2 recognizes a 75 kDa cell surface protein, CD86 (B70/B7-2), expressed primarily on monocytes and activated B cells including lymphoid cells in germinal centers and Epstein-Barr virus transformed B-cell lines. CD86 is the second ligand for CD28 and CTLA-4 and may play an important role in costimulation of T cells in primary immune response. Competitive binding assays demonstrate that, while both IT2.2 and FUN-1 (anti-CD86, Cat. No. 555665) recognize the same molecule, they react with different epitopes. IT2.2 blocks costimulation activity of CD86 in functional studies and blocks binding of human CTLA-4-Ig fusion protein to B70 gene-transfected cells. Profile of Daudi cells analyzed on a BD FACScan™ flowcytometer system .

BD Pharmingen™ Biotin Mouse Anti-Human CD86 - Biotin - Clone IT2.2 - Isotype Mouse IgG2b, κ - Reactivity Hu - 100 Tests

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Clone: IT2-2

Isotype: IgG2b kappa

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Volume d'échantillon: 20 μL

Restrictions: For Research Use only

Stockage

Buffer: Aqueous buffered solution containing BSA and ≤0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed.

Stock: 4 °C

Stockage commentaire: Store undiluted at 4°C.

Références pour anticorps anti-CD86 (Biotin) (ABIN2689422)

Engel, Gribben, Freeman, Zhou, Nozawa, Abe, Nadler, Wakasa, Tedder: "The B7-2 (B70) costimulatory molecule expressed by monocytes and activated B lymphocytes is the CD86 differentiation antigen." dans: Blood, Vol. 84, Issue 5, pp. 1402-7, (1994) (PubMed).

Azuma, Ito, Yagita, Okumura, Phillips, Lanier, Somoza: "B70 antigen is a second ligand for CTLA-4 and CD28." dans: Nature, Vol. 366, Issue 6450, pp. 76-9, (1993) (PubMed).

Nozawa, Wachi, Tominaga, Abe, Wakasa: "A novel monoclonal antibody (FUN-1) identifies an activation antigen in cells of the B-cell lineage and Reed-Sternberg cells." dans: The Journal of pathology, Vol. 169, Issue 3, pp. 309-15, (1993) (PubMed).

Guesdon, Ternynck, Avrameas: "The use of avidin-biotin interaction in immunoenzymatic techniques." dans: The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, Vol. 27, Issue 8, pp. 1131-9, (1979) (PubMed).

Détails sur CD86

Antigène: CD86

Autre désignation: CD86 (CD86 Produits)

Synonymes: anticorps B7-2, anticorps B7.2, anticorps B70, anticorps CD28LG2, anticorps LAB72, anticorps B7, anticorps CLS1, anticorps Cd28l2, anticorps ETC-1, anticorps Ly-58, anticorps Ly58, anticorps MB7, anticorps MB7-2, anticorps TS/A-2, anticorps CD86 molecule, anticorps CD86 antigen, anticorps integrin subunit alpha 2b, anticorps CD86, anticorps Cd86, anticorps Itga2b

Sujet: Synonyms: B70/B7-2

Pathways: TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Immune Effector Process, Activated T Cell Proliferation