CD8B anticorps (Biotin)

N° du produit ABIN2689440

Détail du produit anti-CD8B anticorps (Biotin)

Antigène: CD8B (CD8b Molecule (CD8B))

Reactivité: Rat

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CD8B est conjugé à/à la Biotin

Application: Flow Cytometry (FACS)

Marque: BD Pharmingen™

Attributs du produit: The 341 antibody reacts with the β chain of the CD8 antigen on most thymocytes and a subpopulation of mature T lymphocytes (ie, MHC class I-restricted T cells, including most T suppressor/cytotoxic cells). The CD8 α and β chains (CD8a and CD8b, respectively) form a heterodimer on the surface of most thymocytes and thymus-dependent T suppressor/cytotoxic cells, whereas intestinal intraepithelial lymphocytes, many CD8+ T cells of athymic rats, many activated CD4+ T cells, and most NK cells express CD8a without CD8b. It has been suggested that the expression of the CD8a/CD8b heterodimer is restricted to thymus-derived T lymphocytes. CD8 is an antigen co-receptor on the T cell surface which interacts with MHC class I molecules on antigen-presenting cells. It participates in T-cell activation through its association with the T-cell receptor complex and protein tyrosine kinase lck. Macrophages have also been reported to express CD8 α and β chains, which are involved in signal transduction. The 341 mAb blocks proliferative and cytotoxic in vitro responses of CD8+ effectors to allogeneic cells. Two-color analysis of the expression of CD8b on rat spleen cells. Lewis rat splenocytes were simultaneously stained with FITC-conjugated anti-rat CD8a mAb OX-8 (Cat. no. 554856) and biotinylated mAb 341 (Right panel ), followed by Streptavidin-PE (Cat. no. 554061). Note that the CD8a+CD8b- population represents NK cells. Flow cytometry was performed on a BD FACScan™ flow cytometry system.

BD Pharmingen™ Biotin Mouse Anti-Rat CD8b - Biotin - Clone 341 - Isotype Mouse IgG1, κ - Reactivity Rat - 0.1 mg

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogène: CD8-positive Wistar rat splenic T-cell hybridomas

Clone: 341

Isotype: IgG1 kappa

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Stockage

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed.

Stock: 4 °C

Stockage commentaire: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

Références pour anticorps anti-CD8B (Biotin) (ABIN2689440)

Hirji, Lin, Befus: "A novel CD8 molecule expressed by alveolar and peritoneal macrophages stimulates nitric oxide production." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 158, Issue 4, pp. 1833-40, (1997) (PubMed).

Janeway: "The T cell receptor as a multicomponent signalling machine: CD4/CD8 coreceptors and CD45 in T cell activation." dans: Annual review of immunology, Vol. 10, pp. 645-74, (1992) (PubMed).

Torres-Nagel, Kraus, Brown, Tiefenthaler, Mitnacht, Williams, Hünig: "Differential thymus dependence of rat CD8 isoform expression." dans: European journal of immunology, Vol. 22, Issue 11, pp. 2841-8, (1992) (PubMed).

Bierer, Sleckman, Ratnofsky, Burakoff: "The biologic roles of CD2, CD4, and CD8 in T-cell activation." dans: Annual review of immunology, Vol. 7, pp. 579-99, (1989) (PubMed).

Détails sur CD8B

Antigène: CD8B (CD8b Molecule (CD8B))

Autre désignation: CD8b (CD8B Produits)

Synonymes: anticorps Cd8b, anticorps Ly-3, anticorps Ly-C, anticorps Lyt-3, anticorps CD8B1, anticorps LEU2, anticorps LY3, anticorps LYT3, anticorps P37, anticorps Cd8b1, anticorps CD8b molecule, anticorps CD8 antigen, beta chain 1, anticorps Cd8b, anticorps CD8B, anticorps Cd8b1

Pathways: TCR Signaling