CD8B anticorps

N° du produit ABIN609653

Détail du produit anti-CD8B anticorps

Antigène: CD8B (CD8b Molecule (CD8B))

Reactivité: Rat

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CD8B est non-conjugé

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro))

Specificité: The mouse monoclonal antibody 341 (also known as 34.1) recognizes rat CD8b, the 32-34 kDa beta chain of the CD8 coreceptor (extracellular epitope), expressed on T cell subsets and some other cell types, such as macrophages.

Réactivité croisée (Details): Rat

Purification: Purified by protein-A affinity chromatography.

Pureté: > 95 % (by SDS-PAGE)

Immunogène: CD8 positive Wistar rat splenic T cell hybridomas

Clone: 341

Isotype: IgG1 kappa

Information d'application

Indications d'application: Flow cytometry: Recommended dilution: 1.5 μg/mL.

Restrictions: For Research Use only

Stockage

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Do not freeze.

Stock: 4 °C

Stockage commentaire: Store at 2-8°C. Do not freeze.

Références pour anticorps anti-CD8B (ABIN609653)

Rossi, Del Principe, Rossi, Irno Consalvo, Luciano, Zucchetto, Bulian, Bomben, Dal Bo, Fangazio, Benedetti, Degan, Gaidano, Del Poeta, Gattei: "Prognostic impact of ZAP-70 expression in chronic lymphocytic leukemia: mean fluorescence intensity T/B ratio versus percentage of positive cells." dans: Journal of translational medicine, Vol. 8, pp. 23, (2010) (PubMed).

Vroblova, Vrbacky, Hrudkova, Jankovicova, Schmitzova, Maly, Krejsek, Smolej: "Significant change in ZAP-70 expression during the course of chronic lymphocytic leukemia." dans: European journal of haematology, Vol. 84, Issue 6, pp. 513-7, (2010) (PubMed).

Mabarrack, Turner, Mayrhofer: "Recent thymic origin, differentiation, and turnover of regulatory T cells." dans: Journal of leukocyte biology, Vol. 84, Issue 5, pp. 1287-97, (2008) (PubMed).

Nohara, Kulka, Déry, Wills, Hirji, Gilchrist, Befus: "Regulation of CD8 expression in mast cells by exogenous or endogenous nitric oxide." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 167, Issue 10, pp. 5935-9, (2001) (PubMed).

Ellerman, Like: "Islet cell membrane antigens activate diabetogenic CD4+ T-cells in the BB/Wor rat." dans: Diabetes, Vol. 48, Issue 5, pp. 975-82, (1999) (PubMed).

Lin, Hirji, Nohara, Stenton, Gilchrist, Befus: "Mast cells express novel CD8 molecules that selectively modulate mediator secretion." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 161, Issue 11, pp. 6265-72, (1998) (PubMed).

Kraus, Lambracht, Wonigeit, Hünig: "Negative regulation of rat natural killer cell activity by major histocompatibility complex class I recognition." dans: European journal of immunology, Vol. 26, Issue 11, pp. 2582-6, (1997) (PubMed).

Hirji, Lin, Befus: "A novel CD8 molecule expressed by alveolar and peritoneal macrophages stimulates nitric oxide production." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 158, Issue 4, pp. 1833-40, (1997) (PubMed).

Torres-Nagel, Kraus, Brown, Tiefenthaler, Mitnacht, Williams, Hünig: "Differential thymus dependence of rat CD8 isoform expression." dans: European journal of immunology, Vol. 22, Issue 11, pp. 2841-8, (1992) (PubMed).

Détails sur CD8B

Antigène: CD8B (CD8b Molecule (CD8B))

Autre désignation: CD8b (CD8B Produits)

Synonymes: anticorps Cd8b, anticorps Ly-3, anticorps Ly-C, anticorps Lyt-3, anticorps CD8B1, anticorps LEU2, anticorps LY3, anticorps LYT3, anticorps P37, anticorps Cd8b1, anticorps CD8b molecule, anticorps CD8 antigen, beta chain 1, anticorps Cd8b, anticorps CD8B, anticorps Cd8b1

Sujet: CD8b molecule,The CD8b (CD8 beta) subunit of CD8 T cell coreceptor is expressed in CD8 alpha/beta heterodimers on majority of MHC I-restricted conventional T cells and thymocytes and in CD8 alpha/alpha homodimers on subsets of memory T cells, intraepithelial lymphocytes, NK cells, macrophages, mast cells, and dendritic cells. Regulation of CD8 beta level on T cell surface seems to be an important mechanism to control their effector function. Assembly of CD8 alpha/beta but not alpha/alpha dimers is connected with formation or localization to the lipid rafts. Recruiting triggered TCR complexes to these membrane microdomains as well as affinity of TCR to MHC I is modulated by CD8, thereby affecting the functional diversity of the TCR signaling.,Ly-3, Ly-C, Lyt-3

ID gène: 926

UniProt: P10966

Pathways: TCR Signaling