FAS anticorps (Biotin)

N° du produit ABIN2689462

Cité dans 3 publications. Cet anticorps anti-FAS Monoclonal Souris conjugué à Biotin (Clone DX2) (ABIN2689462) détecte spécifiquement FAS dans FACS. L’anticorps est réactif avec des échantillons de Humain, Porc, Macaque rhésus, Chien, Babouin et Cynomolgus.

Aperçu rapide pour FAS anticorps (Biotin) (ABIN2689462)

Antigène: FAS (TNF Receptor Superfamily, Member 6 (FAS))

Reactivité: Humain, Porc, Macaque rhésus, Chien, Babouin, Cynomolgus

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp FAS est conjugé à/à la Biotin

Application: Flow Cytometry (FACS)

Clone: DX2

Détail du produit anti-FAS anticorps (Biotin)

Attributs du produit: Reacts with the 45 kDa transmembrane cell surface Fas or Apo-1 antigen (designated CD95 at the Fifth HLDA Workshop) which is expressed on a variety of normal and neoplastic cells. The Fas/Apo-1 antigen is a polypeptide, which plays a role in the programmed sequence of events leading to cell death, termed apoptosis. The DX2 clone specifically reacts with murine L cells, murine L1210 leukemia cells and murine P815 mastocytoma cells transfected with human Fas cDNA but not with untransfected parental cell lines. Cross-linking with DX2 delivers an apoptotic signal indicating that DX2 recognizes a functional epitope of the CD95 antigen. This antibody is routinely tested by flow cytometric analysis. Other applications were tested during antibody development only or reported in the literature. Profile of peripheral blood lymphocytes analyzed on a FACScan

BD Pharmingen™ Biotin Mouse Anti-Human CD95 - Biotin - Clone DX2 - Isotype Mouse IgG1, κ - Reactivity Hu, Bab, Cyno, Dog, Pig, Rhe - 100 Tests

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Isotype: IgG1 kappa

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Volume d'échantillon: 20 μL

Restrictions: For Research Use only

Stockage

Buffer: Aqueous buffered solution containing BSA and ≤0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed.

Stock: 4 °C

Stockage commentaire: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

Références pour anticorps anti-FAS (Biotin) (ABIN2689462)

Cifone, De Maria, Roncaioli, Rippo, Azuma, Lanier, Santoni, Testi: "Apoptotic signaling through CD95 (Fas/Apo-1) activates an acidic sphingomyelinase." dans: The Journal of experimental medicine, Vol. 180, Issue 4, pp. 1547-52, (1994) (PubMed).

Itoh, Yonehara, Ishii, Yonehara, Mizushima, Sameshima, Hase, Seto, Nagata: "The polypeptide encoded by the cDNA for human cell surface antigen Fas can mediate apoptosis." dans: Cell, Vol. 66, Issue 2, pp. 233-43, (1991) (PubMed).

Guesdon, Ternynck, Avrameas: "The use of avidin-biotin interaction in immunoenzymatic techniques." dans: The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, Vol. 27, Issue 8, pp. 1131-9, (1979) (PubMed).

Détails sur FAS

Antigène: FAS (TNF Receptor Superfamily, Member 6 (FAS))

Autre désignation: CD95

Sujet: Synonyms: Fas/APO-1

Pathways: Signalisation p53, Apoptose, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity