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LGR5 anticorps (N-Term)

LGR5 Reactivité: Humain WB, FACS, IF, BI Hôte: Rat Monoclonal 8F2 unconjugated
N° du produit ABIN2689804
  • Antigène Voir toutes LGR5 Anticorps
    LGR5 (Leucine-Rich Repeat Containing G Protein-Coupled Receptor 5 (LGR5))
    Épitope
    • 16
    • 15
    • 11
    • 7
    • 7
    • 5
    • 4
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    N-Term
    Reactivité
    • 105
    • 57
    • 49
    • 1
    • 1
    Humain
    Hôte
    • 93
    • 13
    Rat
    Clonalité
    • 82
    • 24
    Monoclonal
    Conjugué
    • 50
    • 6
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Cet anticorp LGR5 est non-conjugé
    Application
    • 79
    • 39
    • 22
    • 20
    • 17
    • 17
    • 14
    • 13
    • 9
    • 7
    • 3
    • 1
    • 1
    • 1
    Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF), BioImaging (BI)
    Marque
    BD Pharmingen™
    Réactivité croisée (Details)
    Mouse (Lack of Reactivity Confirmed in Development)
    Attributs du produit
    Lgr5 (leucine-rich-repeat-containing G-protein-coupled receptor 5) is a seven transmembrane-domain receptor that is a target gene for Wnt and marks stem cells in the small intestine, colon, stomach, and hair follicle. Lgr5 was initially identified as a potential stem cell marker due to restricted expression of Lgr5 in the intestinal crypt and labeling of rapidly cycling cells of the colon and intestine. Using both lineage tracing and organoid culture experiments, Lgr5 positive cells are capable of generating all types of the small intestine epithelium hence indicating that Lgr5 marks stem cells of the small intestine and colon. R-spondin growth factors, which are secreted agonists of the Wnt pathway, bind Lgr5. The binding of R-spondins to Lgr5 leads to recruitment of the Frizzled/LRP Wnt receptor complex, which binds to Wnt ligands and leads to downstream Wnt signaling. Lgr5 is up-regulated in colon and ovarian cancers and has been implicated in promotion of tumor growth and metastasis. The 8F2 monoclonal antibody recognizes an epitope in the N-terminal region of Human Lgr5. Western Blot analysis of human LGR5. Lysates from human epithelial kidney cells, HEK293T, that were transiently transfected with empty vector (lane 1) or human LGR5 (lane 2) were probed with Purified Rat anti-human LGR5 monoclonal antibody (

    BD Pharmingen™ Purified Rat anti-Human Lgr5 (N-Terminal) - Purified - Clone 8F2 - Isotype Rat IgG2b, κ - Reactivity Hu, Ms - 0.1 mg
    Purification
    The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
    Immunogène
    Human LGR5 DNA
    Clone
    8F2
    Isotype
    IgG2b kappa
    Top Product
    Discover our top product LGR5 Anticorps primaire
  • Indications d'application
    Immunohistochemistry-formalin (antigen retrieval required), Immunohistochemistry-frozen (Not Recommended)
    Restrictions
    For Research Use only
  • Concentration
    0.5 mg/mL
    Buffer
    Aqueous buffered solution containing ≤0.09 % sodium azide.
    Agent conservateur
    Sodium azide
    Précaution d'utilisation
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Stock
    4 °C
    Stockage commentaire
    Store undiluted at 4°C.
  • Yui, Nakamura, Sato, Nemoto, Mizutani, Zheng, Ichinose, Nagaishi, Okamoto, Tsuchiya, Clevers, Watanabe: "Functional engraftment of colon epithelium expanded in vitro from a single adult Lgr5⁺ stem cell." dans: Nature medicine, Vol. 18, Issue 4, pp. 618-23, (2012) (PubMed).

    Carmon, Gong, Lin, Thomas, Liu: "R-spondins function as ligands of the orphan receptors LGR4 and LGR5 to regulate Wnt/beta-catenin signaling." dans: Proceedings of the National Academy of Sciences of the United States of America, Vol. 108, Issue 28, pp. 11452-7, (2011) (PubMed).

    Merlos-Suárez, Barriga, Jung, Iglesias, Céspedes, Rossell, Sevillano, Hernando-Momblona, da Silva-Diz, Muñoz, Clevers, Sancho, Mangues, Batlle: "The intestinal stem cell signature identifies colorectal cancer stem cells and predicts disease relapse." dans: Cell stem cell, Vol. 8, Issue 5, pp. 511-24, (2011) (PubMed).

    de Lau, Barker, Low, Koo, Li, Teunissen, Kujala, Haegebarth, Peters, van de Wetering, Stange, van Es, Guardavaccaro, Schasfoort, Mohri, Nishimori, Mohammed, Heck, Clevers: "Lgr5 homologues associate with Wnt receptors and mediate R-spondin signalling." dans: Nature, Vol. 476, Issue 7360, pp. 293-7, (2011) (PubMed).

    Barker, Huch, Kujala, van de Wetering, Snippert, van Es, Sato, Stange, Begthel, van den Born, Danenberg, van den Brink, Korving, Abo, Peters, Wright, Poulsom, Clevers: "Lgr5(+ve) stem cells drive self-renewal in the stomach and build long-lived gastric units in vitro." dans: Cell stem cell, Vol. 6, Issue 1, pp. 25-36, (2010) (PubMed).

    Jaks, Barker, Kasper, van Es, Snippert, Clevers, Toftgård: "Lgr5 marks cycling, yet long-lived, hair follicle stem cells." dans: Nature genetics, Vol. 40, Issue 11, pp. 1291-9, (2008) (PubMed).

    Barker, van Es, Kuipers, Kujala, van den Born, Cozijnsen, Haegebarth, Korving, Begthel, Peters, Clevers: "Identification of stem cells in small intestine and colon by marker gene Lgr5." dans: Nature, Vol. 449, Issue 7165, pp. 1003-7, (2007) (PubMed).

  • Antigène
    LGR5 (Leucine-Rich Repeat Containing G Protein-Coupled Receptor 5 (LGR5))
    Autre désignation
    Lgr5 (LGR5 Produits)
    Synonymes
    anticorps lgr5-a, anticorps lgr5a, anticorps FEX, anticorps Gpr49, anticorps GPR49, anticorps GPR67, anticorps GRP49, anticorps HG38, anticorps leucine-rich repeat containing G protein-coupled receptor 5 L homeolog, anticorps leucine rich repeat containing G protein coupled receptor 5, anticorps leucine rich repeat containing G protein-coupled receptor 5, anticorps lgr5.L, anticorps Lgr5, anticorps LGR5
    Sujet
    Synonyms: GPR49, GPR67, HG38
    Poids moléculaire
    ~110 kDa
    Pathways
    Signalisation WNT
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