PCNA anticorps

N° du produit ABIN2689877

Cet anticorps Souris Monoclonal détecte spécifiquement PCNA dans IHC (f), ICS et BI. Il présente une réactivité envers Humain et a été mentionné dans 1 publication.

Aperçu rapide pour PCNA anticorps (ABIN2689877)

Antigène: PCNA (Proliferating Cell Nuclear Antigen (PCNA))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp PCNA est non-conjugé

Application: Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Intracellular Staining (ICS), BioImaging (BI)

Clone: PC10

Détail du produit anti-PCNA anticorps

Marque: BD Pharmingen™

Attributs du produit: The Proliferating Cell Nuclear Antigen (PCNA) was initially identified as a nuclear antigen in proliferating cells and was subsequently described as a subunit for DNA polymerase δ. PCNA protein levels peak during the S-phase of the cell cycle, at which time it forms a complex with the p21 inhibitor. PCNA is almost undetectable in other phases of the cycle. Because of its unique expression, PCNA has been extensively used in studies associating the prognosis of tumor progression and neoplastic proliferation. Human PCNA has been reported to be 262 amino acids with an apparent molecular weight of 36 kDa. Profile of methanol-fixed MOLT-4 cells analyzed on a BD FACScan™ Immunofluorescent staining of HeLa (ATCC CCL-2) cells. Cells were seeded in a 96 well imaging plate (Cat. No. 353219) at ~10,000 cells per well. After overnight incubation, cells were stained using the alcohol perm protocol and the anti-PCNA antibody. The second step reagent was Alexa Fluor® 555 goat anti-mouse IgG (Invitrogen). The image was taken on a BD Pathway™ 855 Bioimager using a 20x objective. This antibody also stained A549 (ATCC CCL-185) and U-2 OS (ATCC HTB-96) cells. The Triton™ X-100 perm protocol is not recommended for use with this antibody. (See Recommended Assay Procedure).

BD Pharmingen™ Purified Mouse Anti-Human PCNA - Purified - Clone PC10 - Isotype Mouse IgG2a, κ - Reactivity Hu - 0.1 mg

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogène: Not Reported

Isotype: IgG2a kappa

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Stockage

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Store undiluted at 4°C.

Références pour anticorps anti-PCNA (ABIN2689877)

Mathews, Bernstein, Franza, Garrels: "Identity of the proliferating cell nuclear antigen and cyclin." dans: Nature, Vol. 309, Issue 5966, pp. 374-6, (1984) (PubMed).

Détails sur PCNA

Antigène: PCNA (Proliferating Cell Nuclear Antigen (PCNA))

Autre désignation: PCNA

Pathways: Telomere Maintenance, Réparation de l'ADN, Mitotic G1-G1/S Phases, DNA Replication, Synthesis of DNA, Autophagy