PCNA anticorps (Biotin)

N° du produit ABIN2689878

Il existe 5+ publications pour ce produit. L’anticorps anti-PCNA Monoclonal Souris est utilisé pour la détection de PCNA dans des échantillons de Humain. Il a été validé pour ICS.

Aperçu rapide pour PCNA anticorps (Biotin) (ABIN2689878)

Antigène: PCNA (Proliferating Cell Nuclear Antigen (PCNA))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp PCNA est conjugé à/à la Biotin

Application: Intracellular Staining (ICS)

Clone: PC10

Détail du produit anti-PCNA anticorps (Biotin)

Attributs du produit: The Proliferating Cell Nuclear Antigen (PCNA) was initially identified as a nuclear antigen in proliferating cells and was subsequently described as a subunit for DNA polymerase δ. PCNA protein levels peak during the S-phase of the cell cycle, at which time it forms a complex with the p21 inhibitor. PCNA is almost undetectable in other phases of the cycle. Because of its unique expression, PCNA has been extensively used in studies associating the prognosis of tumor progression and neoplastic proliferation. Human PCNA has been reported to be 262 amino acids with an apparent molecular weight of 36 kDa. This antibody is routinely tested by flow cytometric analysis. Other applications were tested during antibody development only or reported in the literature. Profile of peripheral blood lymphocytes analyzed on a FACScan

BD Pharmingen™ Biotin Mouse Anti-Human PCNA - Biotin - Clone PC10 - Isotype Mouse IgG2a, κ - Reactivity Hu - 0.1 mg

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Isotype: IgG2a kappa

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Stockage

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed.

Stock: 4 °C

Stockage commentaire: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

Références pour anticorps anti-PCNA (Biotin) (ABIN2689878)

Schlatt, Weinbauer: "Immunohistochemical localization of proliferating cell nuclear antigen as a tool to study cell proliferation in rodent and primate testes." dans: International journal of andrology, Vol. 17, Issue 4, pp. 214-22, (1995) (PubMed).

Landberg, Tan, Roos: "Flow cytometric multiparameter analysis of proliferating cell nuclear antigen/cyclin and Ki-67 antigen: a new view of the cell cycle." dans: Experimental cell research, Vol. 187, Issue 1, pp. 111-8, (1990) (PubMed).

Ogata, Ogata, Nakamura, Tan: "Purification and N-terminal amino acid sequence of proliferating cell nuclear antigen (PCNA)/cyclin and development of ELISA for anti-PCNA antibodies." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 135, Issue 4, pp. 2623-7, (1985) (PubMed).

Mathews, Bernstein, Franza, Garrels: "Identity of the proliferating cell nuclear antigen and cyclin." dans: Nature, Vol. 309, Issue 5966, pp. 374-6, (1984) (PubMed).

Guesdon, Ternynck, Avrameas: "The use of avidin-biotin interaction in immunoenzymatic techniques." dans: The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, Vol. 27, Issue 8, pp. 1131-9, (1979) (PubMed).

Détails sur PCNA

Antigène: PCNA (Proliferating Cell Nuclear Antigen (PCNA))

Autre désignation: PCNA

Pathways: Telomere Maintenance, Réparation de l'ADN, Mitotic G1-G1/S Phases, DNA Replication, Synthesis of DNA, Autophagy