CCL5 anticorps

N° du produit ABIN2689891

Cité dans 2 publications. Cet anticorps anti-CCL5 Monoclonal Souris (Clone 2D5) (ABIN2689891) détecte spécifiquement CCL5 dans ICC. L’anticorps est réactif avec des échantillons de Humain.

Aperçu rapide pour CCL5 anticorps (ABIN2689891)

Antigène: CCL5 (Chemokine (C-C Motif) Ligand 5 (CCL5))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CCL5 est non-conjugé

Application: Immunocytochemistry (ICC)

Clone: 2D5

Détail du produit anti-CCL5 anticorps

Attributs du produit: The 2D5 antibody reacts with human Regulated upon Activation, Normal T cells Expressed and Secreted (RANTES). The immunogen used to generated this hybridoma was recombinant human RANTES protein. Immunocytochemical staining for RANTES. PBMC were isolated from human peripheral blood by density gradient centrifugation and were cultured overnight at 37 °C with LPS (Sigma Cat. No. L-8274, 1 μg / ml) in the presence of BD GolgiStop™ (Cat. No. 554724). The activated cells were harvested and stained by immunocytochemistry using a three-step staining procedure employing a Biotin Goat Anti-Mouse Ig secondary antibody and a Horseradish Peroxidase (HRP) based detection system (Nomarski optics, original magnification 400X).

BD Pharmingen™ Purified Mouse Anti-Human RANTES - Purified - Clone 2D5 - Isotype Mouse IgG1, κ - Reactivity Hu - 0.25 mg

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogène: Recombinant human RANTES protein

Isotype: IgG1 kappa

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Stockage

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Store undiluted at 4°C.

Références pour anticorps anti-CCL5 (ABIN2689891)

Sticherling, Küpper, Koltrowitz, Bornscheuer, Kulke, Klinger, Wilhelm, Kameyoshi, Christophers, Schröder: "Detection of the chemokine RANTES in cytokine-stimulated human dermal fibroblasts." dans: The Journal of investigative dermatology, Vol. 105, Issue 4, pp. 585-91, (1995) (PubMed).

Hsu, Raine, Fanger: "Use of avidin-biotin-peroxidase complex (ABC) in immunoperoxidase techniques: a comparison between ABC and unlabeled antibody (PAP) procedures." dans: The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, Vol. 29, Issue 4, pp. 577-80, (1981) (PubMed).

Détails sur CCL5

Antigène: CCL5 (Chemokine (C-C Motif) Ligand 5 (CCL5))

Autre désignation: RANTES

Pathways: Cellular Response to Molecule of Bacterial Origin, Regulation of G-Protein Coupled Receptor Protein Signaling, Smooth Muscle Cell Migration