IL3RA anticorps

N° du produit ABIN7687616

L’anticorps anti-IL3RA Monoclonal Souris est utilisé pour la détection de IL3RA dans des échantillons de Humain. Il a été validé pour FACS et IF.

Aperçu rapide pour IL3RA anticorps (ABIN7687616)

Antigène: IL3RA (Interleukin 3 Receptor, alpha (IL3RA))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp IL3RA est non-conjugé

Application: Flow Cytometry (FACS), Immunofluorescence (IF)

Clone: 6H6

Détail du produit anti-IL3RA anticorps

Fonction: Mouse monoclonal antibody to CD123

Specificité: Recognizes human CD123

Attributs du produit: Mouse monoclonal antibody to CD123

Purification: The antibody was purified by affinity chromatography.

Immunogène: Native purified human CD123.

Isotype: IgG1

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Protocole: 1. Take 100 μL peripheral blood anticoagulated by EDTA and add to the bottom of 5 mL tube. 2. Add appropriate amount of antibody to the bottom of flow tube mixing with the whole blood, incubate for 30 minutes at room temperature. 3. Add 2 mL RBC lysis buffer, incubate for 10 minutes after mixing, dissolve red blood cells. 4. Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant. 5. Add 2 mL PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 6. Add appropriate amount of fluorescent-labeled anti-mouse IgGs and incubate for 20 minutes away from light at room temperature. 7. Add 2 mL PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 8. Add 0.5 mL PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: Liquid in PBS, pH 7.3, and 0.02 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

Date de péremption: 12 months

Détails sur IL3RA

Antigène: IL3RA (Interleukin 3 Receptor, alpha (IL3RA))

Autre désignation: CD123

Sujet: IL3R, Interleukin-3 receptor subunit alpha, IL-3 receptor subunit alpha, IL-3R subunit alpha, IL-3R-alpha, IL-3RA, CD antigen CD123

ID gène: 3563

UniProt: P26951

Pathways: Signalistation JAK/STAT