Integrin beta 3 anticorps

N° du produit ABIN7687678

Cet anticorps anti-Integrin beta 3 Monoclonal Souris (Clone PM6-13) (ABIN7687678) détecte spécifiquement Integrin beta 3 dans FACS et IF. L’anticorps est réactif avec des échantillons de Humain.

Aperçu rapide pour Integrin beta 3 anticorps (ABIN7687678)

Antigène: Integrin beta 3 (ITGB3)

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Integrin beta 3 est non-conjugé

Application: Flow Cytometry (FACS), Immunofluorescence (IF)

Clone: PM6-13

Détail du produit anti-Integrin beta 3 anticorps

Fonction: Mouse monoclonal antibody to CD61

Specificité: Recognizes human CD61

Attributs du produit: Mouse monoclonal antibody to CD61

Purification: The antibody was purified by affinity chromatography.

Immunogène: Native purified human CD61.

Isotype: IgG1

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Protocole: 1. Take 100 μL peripheral blood anticoagulated by EDTA and add to the bottom of 5 mL tube. 2. Add appropriate amount of antibody to the bottom of flow tube mixing with the whole blood, incubate for 30 minutes at room temperature. 3. Add 2 mL RBC lysis buffer, incubate for 10 minutes after mixing, dissolve red blood cells. 4. Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant. 5. Add 2 mL PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 6. Add appropriate amount of fluorescent-labeled anti-mouse IgGs and incubate for 20 minutes away from light at room temperature. 7. Add 2 mL PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 8. Add 0.5 mL PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: Liquid in PBS, pH 7.3, and 0.02 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

Date de péremption: 12 months

Détails sur Integrin beta 3

Antigène: Integrin beta 3 (ITGB3)

Autre désignation: CD61

Sujet: GP3A, Integrin beta-3, Platelet membrane glycoprotein IIIa, GPIIIa, CD antigen CD61

ID gène: 3690

UniProt: P05106

Pathways: Regulation of G-Protein Coupled Receptor Protein Signaling, Signaling Events mediated by VEGFR1 and VEGFR2, Smooth Muscle Cell Migration, Integrin Complex