WNK1 anticorps

N° du produit ABIN271862

L’anticorps anti-WNK1 Polyclonal Lapin est utilisé pour la détection de WNK1 dans des échantillons de Humain, Souris et Rat. Il a été validé pour WB et IHC (p).

Aperçu rapide pour WNK1 anticorps (ABIN271862)

Antigène: WNK1 (WNK Lysine Deficient Protein Kinase 1 (WNK1))

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp WNK1 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Détail du produit anti-WNK1 anticorps

Specificité: This antibody detects endogenous levels of WNK1 protein. (region surrounding Tyr52)

Réactivité croisée (Details): Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Pureté: > 95 % pure by SDS-PAGE

Information d'application

Indications d'application: ELISA: 1: 10000approx. 1: 20000. WB: 1: 500approx. 1: 1000. IHC: 1: 50approx. 1: 200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 1,0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.2., 0.05 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: DO NOT FREEZE!

Stock: 4 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C.

Détails sur WNK1

Antigène: WNK1 (WNK Lysine Deficient Protein Kinase 1 (WNK1))

Autre désignation: WNK1

Sujet: The protein kinase superfamily contains over a thousand proteins in 57 subfamilies that all share a catalytic core of 250 - 300 amino acids organized in two domains. WNK kinases (With No lysine (K)) are serine-threonine protein kinases that contain a cysteine residue in place of a lysine residue in a family of proteins that traditionally contain a lysine following a short string of hydro-phobic residues. WNK kinases contain a lysine upstream of the traditional position, within a glycine string. This lysine functions as an anchor and orients ATP through interactions with the alpha and beta phosphoryl groups. The catalytic domains of WNK2, WNK3 and WNK4 are 95 % homologous to WNK1. Human WNK1 maps to chromosome 12p13 and encodes a 2382 protein that is primarily expressed in heart, kidney, muscle and distal nephron. Human WNK3 maps to chromosome Xp11.21-p11.23 and encodes a protein that is primarily expressed in brain. Human WNK4 maps to chromosome 17q21-q22 and encodes a 1243 amino acid protein that is expressed in kidney. Aberrant function of WNK kinases and their associated signaling pathways are implicated in hypertension, increased renal salt reabsorption, and impaired K+ and H+ excretion.Synonyms: Erythrocyte 65 kDa protein, KDP, KIAA0344, Kinase deficient protein, PRKWNK1, Protein kinase lysine-deficient 1, Protein kinase with no lysine 1, Serine/threonine-protein kinase WNK1, p65

Poids moléculaire: approx. 230 kDa

ID gène: 65125

NCBI Accession: NP_001171914

UniProt: Q9H4A3