SUMO1 anticorps

N° du produit ABIN271962

Cet anticorps anti-SUMO1 Polyclonal Lapin (ABIN271962) détecte spécifiquement SUMO1 dans WB, IF et IHC (p). L’anticorps est réactif avec des échantillons de Humain, Rat et Souris.

Aperçu rapide pour SUMO1 anticorps (ABIN271962)

Antigène: SUMO1 (Small Ubiquitin Related Modifier Protein 1 (SUMO1))

Reactivité: Humain, Rat, Souris

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp SUMO1 est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Détail du produit anti-SUMO1 anticorps

Specificité: This antibody detects endogenous levels of Sumo1 protein. (region surrounding Ser2)

Réactivité croisée (Details): Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Pureté: > 95 % pure by SDS-PAGE

Information d'application

Indications d'application: ELISA: 1: 20000approx. 1: 40000. WB: 1: 500approx. 1: 1000. IHC: 1: 50approx. 1: 200. IF: 1: 50approx. 1: 200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 1,0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.2., 0.05 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: DO NOT FREEZE!

Stock: 4 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C.

Détails sur SUMO1

Antigène: SUMO1 (Small Ubiquitin Related Modifier Protein 1 (SUMO1))

Autre désignation: SUMO1

Sujet: The small ubiquitin-related modifier (SUMO) proteins, which include SUMO-1, 2, and 3, belong to the ubiquitin-like protein family. Like ubiquitin, the SUMO proteins are synthesized as precursor proteins that undergo processing before conjugation to target proteins. Also, both utilize the E1, E2, and E3 cascade enzymes for conjugation. However, SUMO and ubiquitin differ with respect to targeting. Ubiquitination predominantly targets proteins for degradation, whereas sumoylation targets proteins to a variety of cellular processing, including nuclear transport, transcriptional regulation, apoptosis, and protein stability. The unconjugated SUMO-1, 2, and 3 proteins are approximately 11 kDa in mass and localize to the nuclear membrane, nuclear bodies, and cytoplasm, respectively. SUMO-1 utilizes Ubc9 for conjugation to several target proteins, which include IκBα, MDM2, p53, PML, and RanGap1. SUMO-2 and 3 contribute to a greater percentage of protein modification than does SUMO-1, and unlike SUMO-1, they can form polymeric chains. In addition, SUMO-3 regulates Amyloid β generation and may be critical in the onset or progression of Alzheimer's disease.Synonyms: GAP-modifying protein 1, GMP1, SMT3 homolog 3, SMT3C, SMT3H3, Sentrin, Small ubiquitin-related modifier 1, UBL1, Ubiquitin-homology domain protein PIC1, Ubiquitin-like protein SMT3C, Ubiquitin-like protein UBL1

Poids moléculaire: approx. 12 kDa

ID gène: 7341

NCBI Accession: NP_001005781

UniProt: P63165

Pathways: M Phase, Positive Regulation of Endopeptidase Activity, Protein targeting to Nucleus, Ubiquitin Proteasome Pathway