Rhodopsin anticorps

N° du produit ABIN272076

Cet anticorps anti-Rhodopsin Polyclonal Lapin (ABIN272076) détecte spécifiquement Rhodopsin dans WB et IHC (p).

Aperçu rapide pour Rhodopsin anticorps (ABIN272076)

Antigène: Rhodopsin (RHO)

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Rhodopsin est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Détail du produit anti-Rhodopsin anticorps

Specificité: This antibody detects endogenous levels of Rhodopsin protein. (region surrounding Leu328)

Réactivité croisée (Details): Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Pureté: > 95 % pure by SDS-PAGE

Information d'application

Indications d'application: ELISA: 1: 1000approx. 1: 5000. IHC: 1: 50approx. 1: 200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 1,0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.2., 0.05 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: DO NOT FREEZE!

Stock: 4 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C.

Détails sur Rhodopsin

Antigène: Rhodopsin (RHO)

Autre désignation: Rhodopsin

Classe de substances: Chemical

Sujet: Retinitis pigmentosa is an inherited progressive disease which is a major cause of blindness in western communities. It can be inherited as an autosomal dominant, autosomal recessive, or X linked recessive disorder. In the autosomal dominant form, which comprises about 25?% of total cases, approximately 30?% of families have mutations in the gene encoding the rod photoreceptor specific protein rhodopsin. This is the transmembrane protein which, when photoexcited, initiates the visual transduction cascade. Defects in this gene are also one of the causes of congenital stationary night blindness. Vision involves the conversion of light into electrochemical signals that are processed by the retina and subsequently sent to and interpreted by the brain. The process of converting light to an electrochemical signal begins when the membrane-bound protein, rhodopsin, absorbs light within the retina. Photoexcitation of rhodopsin causes the cytoplasmic surface of the protein to become catalytically active. In the active state, rhodopsin activates transducin, a GTP binding protein. Once activated, transducin promotes the hydrolysis of cGMP by phosphodiesterase (PDE). The decrease of intracellular cGMP concentrations causes the ion channels within the outer segment of the rod or cone to close, thus causing membrane hyperpolarization and, eventually, signal transmission. Rhodopsin's activity is believed to be shut off by its phosphorylation followed by binding of the soluble protein arrestin.Synonyms: OPN2, Opsin-2, RHO

Poids moléculaire: approx. 42 kDa

ID gène: 6010

NCBI Accession: NP_000530

UniProt: P08100

Pathways: Signalisation WNT, Sensory Perception of Sound, Regulation of G-Protein Coupled Receptor Protein Signaling, Phototransduction