WASF1 anticorps

N° du produit ABIN272093

L’anticorps anti-WASF1 Polyclonal Lapin est utilisé pour la détection de WASF1 dans des échantillons de Humain et Souris. Il a été validé pour WB, IF et IHC (p).

Aperçu rapide pour WASF1 anticorps (ABIN272093)

Antigène: WASF1 (WAS Protein Family, Member 1 (WASF1))

Reactivité: Humain, Souris

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp WASF1 est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Détail du produit anti-WASF1 anticorps

Specificité: This antibody detects endogenous levels of WAVE1 protein. (region surrounding Ile119)

Réactivité croisée (Details): Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Pureté: > 95 % pure by SDS-PAGE

Information d'application

Indications d'application: ELISA: 1: 1000approx. 1: 5000. IHC: 1: 50approx. 1: 200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 1,0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.2., 15 mM sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: DO NOT FREEZE!

Stock: 4 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C.

Détails sur WASF1

Antigène: WASF1 (WAS Protein Family, Member 1 (WASF1))

Autre désignation: WASF1 / WAVE1

Sujet: WASP (for Wiskott-Aldrich syndrome protein) and N-WASP are downstream effectors of Cdc42 that are implicated in actin polymerization and cytoskeletal organization. The WASP family also includes VASP (vasodilator-stimulated phosphoprotein) and Mena (for mammalian enabled protein), which accumulate at focal adhesions and are also involved in the regulation of the actin cytoskeleton. The WAVE proteins are related to the WASP family proteins and are likewise involved in mediating actin reorganization downstream of the Rho family of small GTPases. The two protein homologs WAVE1 and WAVE2 specifically regulate membrane ruffling by inducing the formation of actin filament clusters in response to GTP binding and activating Rac. The WAVE proteins mediate this actin polymerization by cooperating with the Arp2/3 complex, a nucleation core, and thereby promoting the formation of actin filaments. WAVE1, which is also designated SCAR (for suppressor of cAR), is expressed primarily in the brain, while WAVE2 is widely expressed with the expression highest in peripheral blood leukocytes.Synonyms: KIAA0269, SCAR1, Verprolin homology domain-containing protein 1, WASP family protein member 1, WAVE-1, Wiskott-Aldrich syndrome protein family member 1

Poids moléculaire: approx. 65 kDa

ID gène: 8936

NCBI Accession: NP_001020105

UniProt: Q92558

Pathways: Signalisation RTK, Regulation of Actin Filament Polymerization