Survivin anticorps

N° du produit ABIN272115

Cet anticorps anti-Survivin Polyclonal Lapin (ABIN272115) détecte spécifiquement Survivin dans WB, IF et IHC (p). L’anticorps est réactif avec des échantillons de Humain et Souris.

Aperçu rapide pour Survivin anticorps (ABIN272115)

Antigène: Survivin (BIRC5) (Baculoviral IAP Repeat-Containing 5 (BIRC5))

Reactivité: Humain, Souris

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Survivin est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Détail du produit anti-Survivin anticorps

Specificité: This antibody detects endogenous levels of Survivin protein. (region surrounding asn111)

Réactivité croisée (Details): Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Pureté: > 95 % pure by SDS-PAGE

Information d'application

Indications d'application: ELISA: 1: 5000approx. 1: 10000. WB: 1: 500approx. 1: 1000. IHC: 1: 50approx. 1: 200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 1,0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH ~7.2, 0.05 % Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: DO NOT FREEZE!

Stock: 4 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C.

Détails sur Survivin

Antigène: Survivin (BIRC5) (Baculoviral IAP Repeat-Containing 5 (BIRC5))

Autre désignation: IAP4 / BIRC5

Sujet: The baculovirus protein p35 inhibits virally-induced apoptosis of invertebrate and mammalian cells and may function to impair the clearing of virally infected cells by the immune system of the host. This is accomplished at least in part by the ability of p35 to block both TNF- and FAS-mediated apoptosis through the inhibition of the ICE family of serine proteases. Two mammalian homologs of baculovirus p35, referred to as inhibitor of apoptosis protein (IAP) 1 and 2, share an amino-terminal baculovirus IAP repeat (BIR) motif and a carboxy-terminal RING finger. Although the c-IAPs do not directly associate with the TNF receptor (TNF-R), they efficiently block TNF-mediated apoptosis through their interaction with the downstream TNF-R effectors, TRAF1 and TRAF2. Additional IAP family members include ILP (for IAP-like protein) and survivin. ILP inhibits activated caspase-3, leading to the resistance of FASmediated apoptosis. Survivin (also designated TIAP) is expressed during the G2/M phase of the cell cycle and associates with microtubules of the mitotic spindle. Increased caspase-3 activity is detected when a disruption of survivinmicrotubule interactions occurs.Synonyms: API4, Apoptosis inhibitor 4, Apoptosis inhibitor survivin, Baculoviral IAP repeat-containing protein 5, Survivin

Poids moléculaire: approx. 20 kDa

ID gène: 332

NCBI Accession: NP_001012270

UniProt: O15392

Pathways: Apoptose, Cycle Cellulaire, Nuclear Hormone Receptor Binding